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Chemical mutagenesis: role in breeding and biofortification of lentil (Lens culinaris Medik) mutant lines

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Abstract

Background

Induced mutagenesis is a quick and effective breeding strategy to enhance genetic variability, an important prerequisite for the genetic improvement of existing lentil cultivars. Lentil is an important cool season food legume with low productivity due to the low yielding potential of existing lentil cultivars. The present study aimed at increasing the yielding potential, resulted in the isolation of six high-yielding mutant lines with dense micronutrients.

Methods and results

Two lentil varieties were treated with different doses of ethyl methanesulphonate, hydrazine hydrate, and sodium azide, followed by phenotypic selection for consecutive three generations. In the M2 generation, six high-yielding mutant lines with stable phenotypes were isolated. The results revealed a substantial increase in mean values for quantitative and physiological traits coupled with a manifold increase in the genotypic coefficient of variation (GCV), heritability (h2), and genetic advance (GA). Correlation analysis revealed that plant yield was significantly and positively influenced (P < 0.001) by fertile branches per plant, pods per plant, and seed weight. Principal component analysis revealed two principal components contributed 63.5 and 62.5% of the total variation in the varieties Pant L-639 and Pant L-406, respectively.

Conclusion

The isolated high-yielding mutant lines with dense micronutrients that serve as rich genetic resources could be subjected to further breeding trials. After attaining yield stability, these might be registered and released as new improved lentil varieties.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

The authors are thankful to the Chairperson, Department of Botany, Aligarh Muslim University, Aligarh, India, for providing basic research facilities.

Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Mutation Breeding Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, Uttar Pradesh, India

    Aamir Raina & Samiullah Khan

  2. Botany Section, Women’s College, Aligarh Muslim University, Aligarh, 202 002, India

    Aamir Raina

  3. Department of Botany, Abdul Ahad Azad Memorial Degree College Bemina, Cluster University Srinagar, 190 018, Srinagar, Jammu and Kashmir, India

    Mohammad Rafiq Wani

  4. Department of Botany, Bahona College, Jorhat, 785 101, Assam, India

    Rafiul Amin Laskar

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  1. Aamir Raina
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  2. Mohammad Rafiq Wani
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  3. Rafiul Amin Laskar
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  4. Samiullah Khan
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Contributions

AR: contributed to designing and performing the experiments, analyzing and assessing data, and drafting and revising the manuscript. MRW and RAL: contributed to analyzing and evaluating data, SK: contributed to the supervision of overall experimentation. All authors reviewed and approved the manuscript.

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Correspondence to Aamir Raina or Mohammad Rafiq Wani.

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Raina, A., Wani, M.R., Laskar, R.A. et al. Chemical mutagenesis: role in breeding and biofortification of lentil (Lens culinaris Medik) mutant lines. Mol Biol Rep 49, 11313–11325 (2022). https://doi.org/10.1007/s11033-022-07678-6

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  • DOI: https://doi.org/10.1007/s11033-022-07678-6

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