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Identification and characterization of stress responsive homeodomain leucine zipper transcription factors in Medicago truncatula

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Abstract

Background

Homeodomain leucine zipper (HD-ZIP) transcription factors play roles in regulating plant development and responses to abiotic stresses; however, how HD-ZIP genes in Medicago truncatula are involved in abiotic stress response remains elusive.

Methods and results

The HD-ZIP I genes in Medicago truncatula were identified and characterized, and their expression patterns in different tissues and under different abiotic stresses were analyzed. A total of 15 Medicago truncatula HD-ZIP I genes were identified and a phylogenetic analysis of HD-ZIP I proteins in Arabidopsis thaliana and Medicago truncatula was conducted. Fifteen HD-ZIP I genes showed diverse tissue preferences. Among them, expressions of MtHB22 and MtHB51 were specially detected in vegetative buds. In addition, they responded to various abiotic stresses, including salinity and osmotic stress and abscisic acid (ABA). For instance, MtHB7 and MtHB12 expression levels were found to be positively associated with salt, osmotic stress and ABA in both shoots and roots, while MtHB13 and MtHB23 were negatively associated with these stresses in Medicago truncatula.

Conclusion

The HD-ZIP I genes in Medicago truncatula are evolutionarily conserved, but also exhibit gene duplication and gene loss events. Differential expression analysis of Medicago truncatula HD-ZIP I genes indicated their crucial roles in abiotic stress responses. Our genome-wide analysis of the HD-ZIP I transcription factor family in Medicago truncatula provided a valuable reference for further research.

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Data availability

The data supporting the results of this article are included within the article and in the provided supplementary files.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 32071865), China Agriculture Research System of MOF and MARA (CARS-34) and Agricultural Science and Technology Innovation Program (ASTIP-IAS14). The funding body played no role in the design of the study, the collection, analysis, and interpretation of the data or the writing of the manuscript.

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Authors and Affiliations

  1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Xiao Li, Yiyao Hou, Fan Zhang, Mingna Li, Junmei Kang, Qingchuan Yang & Ruicai Long

  2. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, People’s Republic of China

    Fengyan Yi

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  1. Xiao Li
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Contributions

XL and RL conceived and designed the experiments. XL and YH performed the experiments. XL, YH, FZ and FY analyzed the data. XL and RL wrote the original draft. XL, RL, JK, ML, and QY reviewed and edited the manuscript. QY and RL provided financial support. All authors read and approved the final manuscript.

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Correspondence to Ruicai Long.

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Li, X., Hou, Y., Zhang, F. et al. Identification and characterization of stress responsive homeodomain leucine zipper transcription factors in Medicago truncatula. Mol Biol Rep 49, 3569–3581 (2022). https://doi.org/10.1007/s11033-022-07197-4

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