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Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice

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Abstract

Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors, most of which have unknown functions. Here we show that the expression of Oshox22 is strongly induced by salt stress, abscisic acid (ABA), and polyethylene glycol treatment (PEG), and weakly by cold stress. Trans-activation assays in yeast and transient expression analyses in rice protoplasts demonstrated that Oshox22 is able to bind the CAAT(G/C)ATTG element and acts as a transcriptional activator that requires both the HD and Zip domains. Rice plants homozygous for a T-DNA insertion in the promoter region of Oshox22 showed reduced Oshox22 expression and ABA content, decreased sensitivity to ABA, and enhanced tolerance to drought and salt stresses at the seedling stage. In contrast, transgenic rice over-expressing Oshox22 showed increased sensitivity to ABA, increased ABA content, and decreased drought and salt tolerances. Based on these results, we conclude that Oshox22 affects ABA biosynthesis and regulates drought and salt responses through ABA-mediated signal transduction pathways.

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Abbreviations

HD-Zip:

Homeodomain-leucine zipper

ABA:

Abscisic acid

PEG:

Polyethylene glycol

GFP:

Green fluorescent protein

RT:

Reverse transcription

PCR:

Polymerase chain reaction

HB:

Homeobox

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Acknowledgments

We acknowledge the support from projects CEDROME (INCO-CT-2005-015468) for PBFO and CML, the National Natural Science Foundation of China (30821007) and the CAS/SAFEA International Partnership Program for Creative Research Teams (20090491019) for SZ and CML, TF-STRESS (QLK3-CT-2000-00328) for AHM, RNA Seed from the Royal Netherlands Academy of Arts and Sciences (KNAW-CEP 08CDP036) for SZ, PBFO and HS, from the Higher Education Commission (HEC) Pakistan to IH and the Netherlands Organization for Scientific Research (NWO; VICI-grant to HB). We thank Francel Verstappen for his technical support at WUR in The Netherlands.

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Authors and Affiliations

  1. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Shuxin Zhang, Li Jiang, Chun-Ming Liu & Pieter B. F. Ouwerkerk

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, China

    Shuxin Zhang & Li Jiang

  3. Institute of Biology, Leiden University, P.O. BOX 9505, 2300 RA, Leiden, The Netherlands

    Shuxin Zhang, Annemarie H. Meijer & Pieter B. F. Ouwerkerk

  4. Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Imran Haider, Wouter Kohlen & Harro Bouwmeester

  5. Department of Molecular Plant Physiology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Henriette Schluepmann

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  1. Shuxin Zhang
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Correspondence to Chun-Ming Liu or Pieter B. F. Ouwerkerk.

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Supplementary material 1 (DOC 33 kb)

11103_2012_9967_MOESM2_ESM.tif

Fig. S1 The copy number of the T-DNA insertion in oshox22-1. Genomic DNA was digested by EcoRI and the HPT cDNA fragment was used as probe in the Southern blot experiment (TIFF 669 kb)

11103_2012_9967_MOESM3_ESM.tif

Fig. S2 Northern blot analysis of Oshox22 expression in transgenic Oshox22 over-expression plants (middle panel). Equal loading of the RNAs was verified by ethidium bromide staining (lower panel). Upper panel: left: phenotypes of the Oshox22-OX plants; right: wild type Zhonghua 11 (ZH11) plant (TIFF 6074 kb)

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Zhang, S., Haider, I., Kohlen, W. et al. Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice. Plant Mol Biol 80, 571–585 (2012). https://doi.org/10.1007/s11103-012-9967-1

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