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Systematic evaluation of the anti-tumor effect of Phellinus linteus polysaccharide in thyroid carcinoma in vitro

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Abstract

Background

Thyroid carcinoma (TC) is the most common malignant tumor of the endocrine system. Phellinus linteus polysaccharide (PLP) physiologically acts as a suitable anti-tumor molecule. In this study, for the first time, we systematically investigated the anti-tumor activity of PLP in TC, aiming to promote the application of PLP in TC treatment.

Methods and results

TPC-1 and SW579 cells were treated with or without PLP. After treatment, MTT and EdU proliferation assays were performed to detect cell growth. Cell cycle was analyzed using flow cytometry. JC-1 staining was used to track change of mitochondrial membrane potential (MMP). Apoptotic cells were stained with annexin V-fluorescein isothiocyanate/propidium iodide and subsequently analyzed using flow cytometry. mCherry-GFP-LC3 was overexpressed in TC cells by lentiviral technology and the autophagosome was observed using confocal laser scanning microscope. Transwell migration and Matrigel invasion assays were performed to elucidate cell metastasis. Finally, underlying molecular mechanisms were investigated using RT-qPCR and western blotting. PLP inhibited cell growth in TC cells, which was attributable to the PLP-induced arrest at G0/G1 phase of cell cycle. PLP decreased the MMP, induced cell apoptosis, and promoted mitochondrial autophagy and endoplasmic reticulum autophagy. Furthermore, PLP may promote cell apoptosis via nuclear factor kappa-B pathway. In addition, PLP also inhibited cell migration and invasion through modulating the expression of epithelial–mesenchymal transition molecules such as E-cadherin, N-cadherin, and Vimentin

Conclusions

PLP exhibits notable anti-tumor activity in TC cells and may be used in TC treatment.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Code availability

Not applicable.

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Acknowledgements

We are very grateful to the Project of Scientific Research Foundation of Chinese Medicine (Grant Number 2020ZA007) for its financial support.

Funding

The study was supported by the Project of Scientific Research Foundation of Chinese Medicine (Grant Number 2020ZA007).

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Authors and Affiliations

  1. Department of Head and Neck Surgery, Center of Otolaryngology-Head and Neck Surgery, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Key Laboratory of Endocrine Gland Diseases of Zhejiang Province, No. 158 Shangtang Road, Xiacheng District, Hangzhou, 310014, China

    Kun Yu, Zhuo Tan & Ying Xin

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  1. Kun Yu
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  2. Zhuo Tan
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  3. Ying Xin
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Contributions

KY and YX conceived and designed the study. YX critically revised the manuscript. KY and ZT performed the experiments, analyzed the data. KY drafted the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ying Xin.

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The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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Yu, K., Tan, Z. & Xin, Y. Systematic evaluation of the anti-tumor effect of Phellinus linteus polysaccharide in thyroid carcinoma in vitro. Mol Biol Rep 49, 2785–2793 (2022). https://doi.org/10.1007/s11033-021-07090-6

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  • DOI: https://doi.org/10.1007/s11033-021-07090-6

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