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Transcriptomics analysis of Daheng broilers reveals that PLIN2 regulates chicken preadipocyte proliferation, differentiation and apoptosis

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Abstract

Background

Intramuscular fat content, an important meat quality trait, strongly affects flavor, juiciness, and tenderness. Sex hormones regulate lipid metabolism, and female hormones stimulate fat deposition, thereby making the female chickens always fatter than males. In this study, the effect of sex on IMF deposition was screened following transcriptomics in chickens.

Methods and results

Results confirmed significantly higher IMF content of 150-day female chickens as compared to the male chickens. The female chickens manifested higher serum TG, LDL-C, and VLDL, and significantly lower HDL-C contents than male chickens. Moreover, differential expression of genes involved in lipid metabolism were obtained in the muscle and liver between female and male chicken, which could partly interpret the possible reasons for the sex-mediated differences of IMF content. Cellular results revealed that inhibition of PLIN2 significantly inhibited chicken preadipocyte proliferation and induces apoptosis of preadipocytes, as well as promoted adipocyte differentiation.

Conclusions

According to our results, PLIN2 may be considered as a molecular marker for poultry meat quality and applying this gene in early breed selection.

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Data availability

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation. All the data are available in the SRA database with accession number PRJNA732829.

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Funding

This research was funded by Sichuan Science and Technology Program (Grant No. 2021YFYZ0031), the Innovation Key Laboratory of Sichuan Province (Grant No. 2017JZ0033) and Key Technology Support Program of Sichuan Province (Grant No. 2018NZDZX0004).

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Authors and Affiliations

  1. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu, 611130, China

    Jingjing Li, Zhongzhen Lin, Donghao Zhang, Li Wang & Yiping Liu

  2. Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, 610066, Sichuan, China

    Chaowu Yang & Xiaosong Jiang

  3. Faculty of Life Sciences, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China

    Peng Ren

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  1. Jingjing Li
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  2. Chaowu Yang
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  3. Peng Ren
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  4. Zhongzhen Lin
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  5. Donghao Zhang
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  7. Li Wang
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  8. Yiping Liu
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Contributions

Conceptualization, JL and CY; methodology, JL and PR; software, JL and ZL; investigation, JL, CY, DZ and LW; resources, CY and XJ; writing—original draft preparation, JL; writing—review and editing, CY, XJ, PR and YL; supervision, XJ and YL; project administration, YL; funding acquisition, YL and CY All authors have read and agreed to the published version of the manuscript.

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Correspondence to Yiping Liu.

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The animal study was reviewed and approved by the Ethics Committee for Animal Experiments of Sichuan agricultural university.

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Li, J., Yang, C., Ren, P. et al. Transcriptomics analysis of Daheng broilers reveals that PLIN2 regulates chicken preadipocyte proliferation, differentiation and apoptosis. Mol Biol Rep 48, 7985–7997 (2021). https://doi.org/10.1007/s11033-021-06831-x

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