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Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck

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Abstract

Liver fatty acid binding protein (L-FABP) is a member of intracellular lipid-binding proteins responsible for the transportation of fatty acids. The expression pattern of duck L-FABP mRNA was examined in this study by quantitative RT-PCR. The results showed that duck L-FABP gene was expressed in many tissues, including heart, lung, kidney, muscle, ovary, brain, intestine, stomach and adipocyte tissues, and highly expressed in liver. Several lipid metabolism-related genes were selected to detect the regulation of L-FABP in duck. The expression of L-FABP and lipoprotein lipase was promoted by oleic acid. The L-FABP knockdown decreased the expression levels of peroxisome proliferator-activated receptor α (PPARα), fatty acid synthase and lipoprotein lipase by 61.1, 42.3 and 53.7 %, respectively (P < 0.05), but had no influences on the mRNA levels of PPARγ and leptin receptor. L-FABP might function through the PPARα to regulate the fat metabolism-related gene expression and play important roles in lipid metabolism in duck hepatocytes.

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Acknowledgments

This study was supported by National 948 Projects, China (No. 2012-Z47) and the Significant Project of Science and Technology of Zhejiang Province, P. R. China (No. 2007C14023).

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Authors and Affiliations

  1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou, 310021, Zhejiang, China

    Jun He, Yong Tian, Jinjun Li, Junda Shen, Zhengrong Tao & Lizhi Lu

  2. College of Animal Sciences, Zhejiang University, 388 Yuhangtang Road, Hangzhou, 310058, Zhejiang, China

    Jun He, Yan Fu & Dong Niu

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  1. Jun He
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  2. Yong Tian
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  3. Jinjun Li
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Correspondence to Dong Niu or Lizhi Lu.

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He, J., Tian, Y., Li, J. et al. Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck. Mol Biol Rep 40, 189–195 (2013). https://doi.org/10.1007/s11033-012-2048-3

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  • DOI: https://doi.org/10.1007/s11033-012-2048-3

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