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Comparison of the transcriptomic responses of two Chrysanthemum morifolium cultivars to low light

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Abstract

Background

Low light is a primary regulator of chrysanthemum growth. Our aim was to analyse the different transcriptomic responses of two Chrysanthemum morifolium cultivars to low light.

Methods and results

We conducted a transcriptomic analysis of leaf samples from the ‘Nannonggongfen’ and ‘Nannongxuefeng’ chrysanthemum cultivars following a 5-day exposure to optimal light (70%, control [CK]) or low-light (20%, LL) conditions. Gene Ontology (GO) classification of upregulated genes revealed these genes to be associated with 11 cellular components, 9 molecular functions, and 15 biological processes, with the majority being localized to the chloroplast, highlighting the role of chloroplast proteins as regulators of shading tolerance. Downregulated genes were associated with 11 cellular components, 8 molecular functions, and 16 biological processes. Heat map analyses suggested that basic helix–loop–helix domain genes and elongation factors were markedly downregulated in ‘Nannongxuefeng’ leaves, consistent with the maintenance of normal stem length, whereas no comparable changes were observed in ‘Nanonggongfen’ leaves. Subsequent qPCR analyses revealed that phytochrome-interacting factors and dormancy-associated genes were significantly upregulated under LL conditions relative to CK conditions, while succinate dehydrogenase 1, elongated hypocotyls 5, and auxin-responsive gene of were significantly downregulated under LL conditions.

Conclusions

These findings suggest that LL plants were significantly lower than those of the CK plants. Low-light tolerant chrysanthemum cultivars may maintain reduced indole-3-acetic acid (IAA) and elongation factor expression as a means of preventing the onset of shade-avoidance symptoms.

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Funding

This work was funded by the National Natural Science Foundation of China (31902066), Scientific and Technological Projects of Henan Province (182102110241), and the Henan Provincial Natural Science Foundation, China (182300410058). We would like to thank the Nanjing Agricultural University for providing the chrysanthemum materials and the Program for Science & Technology Innovative Research Team in University of Henan Province (21IRTSTHN025).

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Authors and Affiliations

  1. Henan Provincial Key University Laboratory of Plant-Microbe Interactions, College of Biology and Food, Shangqiu Normal University, Shangqiu, 476000, China

    Shuang Han, Qingchen Zhang, Haojie Wang & Dongli Pei

Authors
  1. Shuang Han
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  2. Qingchen Zhang
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  3. Haojie Wang
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  4. Dongli Pei
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Contributions

Performed the experiments: SH and QZ; analyzed the data: HW; prepared and wrote the manuscript: SH; conceived and designed the experiment: DP.

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Correspondence to Dongli Pei.

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The authors in this study declared no conflict of any interest.

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Han, S., Zhang, Q., Wang, H. et al. Comparison of the transcriptomic responses of two Chrysanthemum morifolium cultivars to low light. Mol Biol Rep 48, 7293–7301 (2021). https://doi.org/10.1007/s11033-021-06729-8

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  • DOI: https://doi.org/10.1007/s11033-021-06729-8

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