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Overexpression of Ks-type dehydrins gene OeSRC1 from Olea europaea increases salt and drought tolerance in tobacco plants

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Abstract

Agricultural production is greatly affected by environmental stresses, such as cold, drought and high-salinity. It is possible to produce tolerant genotypes by transferring genes encoding protective proteins or enzymes from other organisms. In this regard, the current study was aimed to clone a novel OeSRC1 gene identified during the transcriptome profiling of olives (Olea europaea L.) and to investigate the function of this gene in tobacco plants. Functional evaluation of OeSRC1 gene in putative transgenic tobacco plants were carried out under drought, cold and salt stress conditions by using molecular and biochemical tools. It was observed that the transgenic tobacco plants exhibited higher seed germination and survival rates, better root and shoot growth under cold, salt and drought stress treatments compared to wild type plants. Our results also demonstrated that, under stress conditions, transgenic plants accumulated more free proline while no significant changes were observed regarding electrolyte leakage. Ascorbate peroxidase activity of OeSRC1-overexpressing plants was higher than those of the WT plants under different stress conditions. The overall results demonstrate the explicit role of OeSRC1 gene in conferring multiple abiotic stress tolerance at the whole-plant level. The multifunctional role of olive OeSRC1 gene looks good to enhance environmental stress tolerance in diverse plants.

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Acknowledgements

This research was supported by Research Fund of Ondokuz Mayıs University PYO.ZRT.1904.15.006.

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Authors and Affiliations

  1. Laboratory of Plant Cell Technology, Graduate School of Horticulture, Faculty of Horticulture, Chiba University, 648 Matsudo Matsudo-shi, Chiba, 271-8510, Japan

    Samuel Aduse Poku

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Ondokuz Mayıs University, 55200, Samsun, Turkey

    Zafer Seçgin & Musa Kavas

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  1. Samuel Aduse Poku
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  2. Zafer Seçgin
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  3. Musa Kavas
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Contributions

SAP: Carried out the experiment, analyzed the data. ZS: helped in performing the experiment. MK: conceived the idea, planned the experiments, supervised the experiments, wrote the manuscript.

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Correspondence to Musa Kavas.

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11033_2019_5008_MOESM1_ESM.jpg

Supplementary material 1 (JPEG 248 kb) Fig. S1. Average root length (cm) of WT and Line 4 plantlets. Plantlets germinated and incubated on MS medium containing 200 mM NaCl and 400 mM Mannitol for 8 weeks

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Poku, S.A., Seçgin, Z. & Kavas, M. Overexpression of Ks-type dehydrins gene OeSRC1 from Olea europaea increases salt and drought tolerance in tobacco plants. Mol Biol Rep 46, 5745–5757 (2019). https://doi.org/10.1007/s11033-019-05008-x

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  • DOI: https://doi.org/10.1007/s11033-019-05008-x

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