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Differential expression of ferritin genes in response to abiotic stresses and hormones in pear (Pyrus pyrifolia)

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Abstract

In this study, the expression patterns of four ferritin genes (PpFer1, PpFer2, PpFer3, and PpFer4) in pear were investigated using quantitative real-time PCR. Analysis of tissue-specific expression revealed higher expression level of these genes in leaves than in other tested tissues. These ferritin genes were differentially expressed in response to various abiotic stresses and hormones treatments. The expression of ferritin wasn’t affected by Fe(III)-citrate treatment. Abscisic acid significantly enhanced the expression of all four ferritin genes, especially PpFer2, followed by N-benzylyminopurine, gibberellic acid, and indole-3-acetic acid. The expression peaks of PpFer1 and PpFer3 in leaves appeared at 6, 6, and 12 h, respectively, after pear plant was exposed to oxidative stress (5 mM H2O2), salt stress (200 mM NaCl), and heat stress (40°C). A significant increase in PpFer4 expression was detected at 6 h after salt stress or heat stress. The expression of ferritin genes was not altered by cold stress. These results suggested that ferritin genes might be functionally important in acclimation of pear to salt and oxidative stresses. Hormone treatments had no significant effect on expression of ferritin genes compared to abiotic stresses. This showed accumulation of ferritin genes could be operated by different transduction pathways under abiotic stresses and hormones treatments.

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Acknowledgments

The authors thank all lab members, particularly, Zhaoguo Tong, for their assistance and suggestions when they were conducting the research. They are highly grateful to Dr. Xia Ye for her critical comments when they were preparing this manuscript. This work was supported by National and Jiangsu Provincial Program for High Technology Research and Development in China (Nos.: 2006AA100108, BG2006313).

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Authors and Affiliations

  1. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Li Xi, Kuanyong Xu, Yushan Qiao, Shenchun Qu & Zhen Zhang

  2. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    Wenhao Dai

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  1. Li Xi
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  2. Kuanyong Xu
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  3. Yushan Qiao
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Correspondence to Zhen Zhang.

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Xi, L., Xu, K., Qiao, Y. et al. Differential expression of ferritin genes in response to abiotic stresses and hormones in pear (Pyrus pyrifolia). Mol Biol Rep 38, 4405–4413 (2011). https://doi.org/10.1007/s11033-010-0568-2

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