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Differential expression of iron-sulfur cluster biosynthesis genes during peach flowering

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Iron is required for the Fe-S cluster assembly which occurs in chloroplasts, mitochondria, and cytosol and here we characterized 44 Fe-S cluster biosynthesis genes and investigated their expression profiles during different peach flowering stages. Quantitative real-time PCR analysis shows that the highest expression of most peach Fe-S cluster biosynthesis genes appeared in the full bloom stage. Also, the highest Fe accumulation occurred in the full bloom stage followed by beginning bloom, petal fall, and bud swell stages. Activities of nitrite reductase (NiR) and succinate dehydrogenase (SDH) were closely correlated to the flower Fe content, whereas the aconitase (ACO) activity kept steady during the whole flowering process. Moreover, shading treatment significantly reduced Fe accumulation and NiR, SDH, and ACO activities of the full blooming flowers. Seventeen Fe-S cluster biosynthesis genes were down-regulated in response to a shading treatment. In particular, plastid sulfur mobilization genes were sensitive to the shading treatment.

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Abbreviations

ACO:

aconitase

CIA:

cytosolic iron-sulfur cluster assembly

FS:

flowering stage

ICP-AES:

inductively coupled plasma atomatic emission spectrometry

ISC:

iron-sulfur cluster

NiR:

nitrite reductase

RT-qPCR:

real-time quantitative PCR

SDH:

succinate dehydrogenase

SUF:

sulfur mobilization

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Authors and Affiliations

  1. Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P.R. China

    Z. -Z. Song, B. -B. Zhang, C. -H. Zhang, R. -J. Ma & M. -L. Yu

  2. Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing, 210014, P.R. China

    Z. -Z. Song, B. -B. Zhang, C. -H. Zhang, R. -J. Ma & M. -L. Yu

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  1. Z. -Z. Song
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  2. B. -B. Zhang
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  3. C. -H. Zhang
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  4. R. -J. Ma
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  5. M. -L. Yu
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Correspondence to M. -L. Yu.

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Acknowledgments: This work was supported by grants from the National Natural Science Foundations of China (31501743), the China Agriculture Research System (CARS-31), the China Postdoctoral Science Foundation (2015M571713), and the Jiangsu Agriculture Science and Technology Innovation Fund (CX(14)2015).

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Song, Z.Z., Zhang, B.B., Zhang, C.H. et al. Differential expression of iron-sulfur cluster biosynthesis genes during peach flowering. Biol Plant 60, 79–85 (2016). https://doi.org/10.1007/s10535-015-0577-z

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  • DOI: https://doi.org/10.1007/s10535-015-0577-z

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