Abstract
Plant vacuolar H+-ATPase (V-ATPase) plays an important role in response to different adverse environmental conditions. In the present study, we cloned and characterized a V-ATPase c subunit gene (ThVHAc1) from Tamarix hispida. The deduced ThVHAc1 amino acid sequence lacks a signal peptide and ThVHAc1 is a highly hydrophobic protein with four transmembrane regions. A transient expression assay showed that the ThVHAc1-GFP fusion protein is expressed on onion epidermal endomembrane cells. Real-time RT-PCR demonstrated that ThVHAc1 gene expression was induced by NaCl, NaHCO3, PEG and CdCl2 stress in T. hispida roots, stems and leaves. Exogenous application of abscisic acid (ABA) also stimulated ThVHAc1 transcript levels in the absence of stress, suggesting that ThVHAc1 is involved in ABA-dependent stress signaling pathway. Furthermore, the transgenic yeast expressing ThVHAc1 increased salt, drought, ultraviolet (UV), oxidative, heavy metal, cold and high temperature tolerance. Our results suggested that the ThVHAc1 gene from T. hispida serves a stress tolerance role in the species.
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Acknowledgements
This work has been supported by China postdoctoral Science Foundation (no.20090450943), the Fundamental Research Funds for the Central Universities (DL09BA06), New Century Excellent Talents in University (NCET), Ministry of Education, China (NCET-08-0751), and Natural Science Foundation of Heilongjiang province (QC07C56).
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Gao, C., Wang, Y., Jiang, B. et al. A novel vacuolar membrane H+-ATPase c subunit gene (ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae . Mol Biol Rep 38, 957–963 (2011). https://doi.org/10.1007/s11033-010-0189-9
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DOI: https://doi.org/10.1007/s11033-010-0189-9