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A polymorphism in the visfatin gene promoter is related to decreased plasma levels of inflammatory markers in patients with coronary artery disease

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Abstract

Visfatin, a newly identified proinflammatory adipokine, has been linked to coronary artery disease (CAD). The −1535C>T polymorphism (rs61330082) located in the visfatin gene promoter is reportedly associated with proinflammatory status. However, it is unclear whether this polymorphism correlates with plasma levels of inflammatory markers including visfatin, hs-CRP, IL-6 and TNF-α in CAD patients. The present study was to investigate the potential association of the −1535C>T polymorphism with plasma levels of visfatin, IL-6, C reactive protein (hs-CRP) and TNF-α in patients with CAD. We conducted a hospital based study with 171 CAD patients to examine the association between the −1535C>T polymorphism and plasma levels of visfatin, hs-CRP, IL-6 and TNF-α. Plasma visfatin levels were markedly different between patients with stable angina pectoris (SAP, 11.91 ± 0.70 ng/l) and those with unstable angina pectoris (UAP, 17.49 ± 0.20 ng/l) or acute myocardial infarction (AMI, 16.63 ± 0.22 ng/l; SAP versus UAP or AMI, P < 0.05). Compared with the CC genotype, variant genotypes CT and TT correlated with significantly lower levels of visfatin, hs-CRP, IL-6 and TNF-α in the SAP group (P < 0.05), with lower levels of hs-CRP and IL-6 in the UAP group (P < 0.05), and with lower levels of visfatin in the AMI group (P < 0.05) after adjustment for age, gender, smoking, hypertension, diabetes, dyslipidemia and medication. Our results suggest that the −1535C>T polymorphism is associated with decreased plasma levels of inflammatory markers in CAD patients, reflecting that this polymorphism might provide a useful marker for predicting the development of CAD events.

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References

  1. Rosamond W, Flegal K, Furie K et al (2008) Heart disease and stroke statistics—2008 update: a report from the American. Circulation 117(4):e25–e146

    Article  PubMed  Google Scholar 

  2. Hansson GK (2005) Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med 352:1685–1695

    Article  CAS  PubMed  Google Scholar 

  3. Libby P, Sasiela W (2006) Plaque stabilization: can we turn theory into evidence? Am J Cardiol 98((11A)):26P–33P

    Article  CAS  PubMed  Google Scholar 

  4. Samal B, Sun Y, Stearns G et al (1994) Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor. Mol Cell Biol 14(2):1431–1437

    CAS  PubMed  Google Scholar 

  5. Oki K, Yamane K, Kamei N et al (2007) Circulating visfatin level is correlated with inflammation, but not with insulin resistance. Clin Endocrinol 67:796–800

    Article  CAS  Google Scholar 

  6. Moschen AR, Kaser A, Enrich B et al (2007) Visfatin, an adipocytokine with proinflammatory and immunomodulating properties. J Immunol 178:1748–1758

    CAS  PubMed  Google Scholar 

  7. Dahl TB, Yndestad A, Skjelland M et al (2007) Increased expression of visfatin in macrophages of human unstable carotid and coronary atherosclerosis: possible role in inflammation and plaque destabilization. Circulation 115(8):972–980

    Article  CAS  PubMed  Google Scholar 

  8. Liu SW, Qiao SB, Yuan JS, Liu DQ (2009) Association of plasma visfatin levels with inflammation, atherosclerosis, and acute coronary syndromes in humans. Clin Endocrinol 71(2):202–207

    Article  CAS  Google Scholar 

  9. Zhang YY, Gottardo L, Thompson R et al (2006) A visfatin promoter polymorphism is associated with low-grade inflammation and type 2 diabetes. Obesity 14:2119–2126

    Article  CAS  PubMed  Google Scholar 

  10. Ye SQ, Simon BA, Maloney JP et al (2005) Pre-B-cell colony enhancing factor as a potential novel biomarker in acute lung injury. Am J Respir Crit Care Med 171:361–370

    Article  PubMed  Google Scholar 

  11. Tokunaga A, Miura A, Okauchi Y et al (2008) The −1535 promoter variant of the visfatin gene is associated with serum triglyceride and HDL-cholesterol levels in Japanese subjects. Endocr J 55:205–212

    Article  CAS  PubMed  Google Scholar 

  12. Jian WX, Luo TH, Gu YY et al (2006) The visfatin gene is associated with glucose and lipid metabolism in a Chinese population. Diabet Med 23:967–973

    Article  CAS  PubMed  Google Scholar 

  13. Yan JJ, Tang NP, Tang JJ et al (2010) Genetic variant in visfatin gene promoter is associated with decreased risk of coronary artery disease in a Chinese population. Clin Chim Acta 411(1-2):26–30

    Article  CAS  PubMed  Google Scholar 

  14. Santamore WP, Kahl FR, Kutcher MA et al (1988) A microcomputer based automated, quantitative coronary angiographic analysis system. Ann Biomed Eng 16:367–377

    Article  CAS  PubMed  Google Scholar 

  15. Ehara S, Kobayashi Y, Yoshiyama M et al (2004) Spotty calcification typifies the culprit plaque in patients with acute myocardial infarction: an intravascular ultrasound study. Circulation 110(22):3424–3429

    Article  PubMed  Google Scholar 

  16. Wang B, Pan J, Wang L et al (2006) Associations of plasma 8-isoprostane levels with the presence and extent of coronary stenosis in patients with coronary artery disease. Atherosclerosis 184:425–430

    Article  CAS  PubMed  Google Scholar 

  17. Chen Z, Qian Q, Ma G, Wang J et al (2009) A common variant on chromosome 9p21 affects the risk of early-onset coronary artery disease. Mol Biol Rep 36(5):889–893

    Article  CAS  PubMed  Google Scholar 

  18. Bressler J, Folsom AR, Couper DJ et al (2010) Genetic variants identified in a European genome-wide association study that were found to predict incident coronary heart disease in the atherosclerosis risk in communities study. Am J Epidemiol. 171(1):14–23

    Article  PubMed  Google Scholar 

  19. Pasalić D, Marinković N, Grsković B et al (2009) C-reactive protein gene polymorphisms affect plasma CRP and homocysteine concentrations in subjects with and without angiographically confirmed coronary artery disease. Mol Biol Rep 36(4):775–780

    Article  PubMed  Google Scholar 

  20. Lucas AR, Korol R, Pepine CJ (2006) Inflammation in atherosclerosis: some thoughts about acute coronary syndromes. Circulation 113(17):e728–e732

    Article  CAS  PubMed  Google Scholar 

  21. Fukuhara A, Matsuda M, Nishizawa M et al (2005) Visfatin: a protein secreted by visceral fat that mimics the effects of insulin. Science 307:426–430

    Article  CAS  PubMed  Google Scholar 

  22. Ognjanovic S, Bao S, Yamamoto SY et al (2001) Genomic organization of the gene coding for human pre-B-cell colony enhancing factor and experssion in human fetal membranes. J Mol Endocrinol 26(2):107–117

    Article  CAS  PubMed  Google Scholar 

  23. Ognjanovic S, Bryant-Greenwood GD (2002) Pre-B-cell colony-enhancing factor, a novel cytokine of human fetal membranes. Am J Obstet Gynecol 187(4):1051–1058

    Article  CAS  PubMed  Google Scholar 

  24. Adya R, Tan BK, Punn A, Chen J, Randeva HS (2008) Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis. Cardiovasc Res 78(2):356–365

    Article  CAS  PubMed  Google Scholar 

  25. Mizuno K, Satomura K, Miyamoto A et al (1992) Angioscopic evaluation of coronary artery thrombi in acute coronary syndromes. N Engl J Med 326(5):287–291

    Article  CAS  PubMed  Google Scholar 

  26. Fuster V, Badimon L, Badimon JJ et al (1992) The pathologenesis of coronary artery disease and the acute coronary syndrome. N Engl J Med 326(4):242–250

    Article  CAS  PubMed  Google Scholar 

  27. Kuhlmann MK, Yoshino M, Levin NW (2004) Differences in cardiovascular mortality rates among hemodialysis patients in the United States and Japan: the importance of background cardiovascular mortality. Hemodial Int 8(4):394–399

    Article  PubMed  Google Scholar 

  28. Morange PE, Tregouet DA, Godefroy T et al (2008) Polymorphisms of the tumor necrosis factor-alpha (TNF) and the TNF-alpha converting enzyme (TACE/ADAM17) genes in relation to cardiovascular mortality: the AtheroGene study. J Mol Med 86(10):1153–1161

    Article  CAS  PubMed  Google Scholar 

  29. Liu P, Li H, Cepeda J et al (2009) Critical role of PBEF expression in pulmonary cell inflammation and permeability. Cell Biol Int 33(1):19–30

    Article  CAS  PubMed  Google Scholar 

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Acknowledgment

This project was supported by the grant from the National Natural Science Foundation of China (No. 30871078).

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Authors and Affiliations

  1. Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, China

    Lian-Sheng Wang, Jian-Jun Yan, Jun Zhu, Qi-Ming Wang, Jian-Jin Tang, Ming-Wei Wang, En-Zhi Jia, Zhi-Jian Yang & Jun Huang

  2. National Shanghai Center for New Drug Safety Evaluation and Research, Shanghai Institute of Pharmaceutical Industry, 199 Guoshoujing Road, Zhangjiang Hi-Tech Park, Pudong, Shanghai, 201203, China

    Na-Ping Tang

  3. Division of Cardiology, Ma-anshan Municipal People’s Hospital, Anhui, 243000, China

    Yue-Song Wang

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  1. Lian-Sheng Wang
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Correspondence to Lian-Sheng Wang.

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Wang, LS., Yan, JJ., Tang, NP. et al. A polymorphism in the visfatin gene promoter is related to decreased plasma levels of inflammatory markers in patients with coronary artery disease. Mol Biol Rep 38, 819–825 (2011). https://doi.org/10.1007/s11033-010-0171-6

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  • DOI: https://doi.org/10.1007/s11033-010-0171-6

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