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Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content

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Abstract

Porcine chromosome 6 (SSC6) has been reported to have QTL affecting intramuscular fat content (IMF) in multiple populations. The objective of this study was to investigate the effect of FABP3 and LEPR genetic variations as well as their mRNA expression on the IMF trait in a three-generation of Korean native pig and Yorkshire crossed animals. Several polymorphisms of the FABP3 (HinfI, HaeIII and HinfI*) were significantly associated with moisture, tenderness and flavor score (P < 0.05), and were used to construct haploytpes: haplotype 1 (-TCT-) increased the marbling and intramuscular fat content, however, haplotype 2 (-CCT-) decreased tenderness. The LEPR AvaII polymorphism showed significant association with moisture, intramuscular fat, cholesterol and flavor score (P < 0.05). The linkage analyses with six microsatellites mapped FABP3 gene in the interval between the markers Sw1129 and S0228 (Sw1129—11.7 cM—FABP3—9.1 cM—S0228), and the LEPR gene between the markers S0121 and Sw322 (S0121—7.5 cM—LEPR—28.5 cM—Sw322). QTL mapping suggested a significant QTL affecting Moisture (83 cM) and IMF (84 cM) located close to marker S0228. The gene expression results showed that in the loin muscle, both of the FABP3 and LEPR genes showed significantly higher expression in pigs with higher IMF%, however, in the backfat, only FABP3 showed differential expression between these two groups of pigs (significantly higher expression in pigs with lower IMF%) (P < 0.05). In the liver, both of these two genes did not show any difference between the high and low IMF% groups.

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Acknowledgements

This work was supported by a grant titled “Genetic improvement maximization of Korean native pigs using marker-assisted selection, and construction of commercial line production system” from the BioGreen 21 Program of the Korea Rural Development Administration.

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Authors and Affiliations

  1. Department of Animal Science, Chungbuk National University, 12 Gaeshin-dong, Heungduk-ku, Cheongju, Chungbuk, 361-763, Korea

    Xiaoping Li, Sang-Wook Kim, Jung-Suck Choi, Yang-Il Choi & Kwan-Suk Kim

  2. Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong (Central China) Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Xiaoping Li

  3. School of Biotechnology, Yeungnam University, Gyeongsan, Korea

    Yoon-Mi Lee & Jong-Joo Kim

  4. College of Life and Environmental Sciences, Korea University, Seoul, Korea

    Cheol-Koo Lee

  5. National Institute of Animal Science, Suwon, Korea

    Bong-Hwan Choi & Tae-Hun Kim

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  1. Xiaoping Li
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Li, X., Kim, SW., Choi, JS. et al. Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content. Mol Biol Rep 37, 3931–3939 (2010). https://doi.org/10.1007/s11033-010-0050-1

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