Abstract
The purpose of this study was to detect genetic variation in the porcine H-FABP gene, a candidate gene for meat quality traits in pigs. Lambda phages containing the porcine H-FABP gene were isolated by plaque hybridization with human H-FABP cDNA. The coding and flanking intronic sequences of the porcine H-FABP gene were determined as well as 1.6 kb of the 5 ′ upstream region. The various potential regulatory sequences in this region are in accordance with the function and expression of the protein in muscle and mammary tissue. Furthermore, comparison with the homolog region of the mouse identified a highly conserved 13-bp element (CTTCCT [A/C] TTTCGG) that may be involved in regulation of expression. The porcine H-FABP gene was localized on Chromosome (Chr) 6 by porcine sequence-specific PCR on DNA from a pig/rodent cell hybrid panel. In addition, part of the H-FABP gene was screened for genetic variation by PCR-RFLP analysis. Three PCR-RFLPs were detected, one in the upstream region (HinfI) and two in the second intron (HaeIII and MspI). In most pig breeds the corresponding alleles have a variable distribution, possibly a consequence of selective breeding. This genetic variation will enable us to investigate the role of the H-FABP locus in porcine production and meat quality traits.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bahary N, Zorich G, Pachter JE, Leibel RL, Friedman JM (1991) Molecular genetic linkage maps of mouse chromosomes 4 and 6. Genomics 11, 33–47
Billich S, Wissel T, Kratzin H, Hahn U, Hagenhoff B, Lezius AG, Spener F (1988) Cloning of a full length complementary DNA for fatty acidbinding protein from bovine heart. Eur J Biochem 175, 549–556
Binas B, Spitzer E, Zschiesche W, Erdmann B, Kurtz A, Mueller T, Niemann C, Blenau W, Grosse R (1992) Hormonal induction of functional differentiation and mammary derived growth inhibitor expression in cultured mouse mammary gland expiants. In Vitro Cell Dev Biol 28A, 625–634
Bohmer F, Kraft R, Otto A, Wernstadt C, Hellmann U, Kurtz A, Muller T, Rohde K, Etzold G, Lehmann W, Langen P, Heldin C-H, Grosse R (1987) Identification of a polypeptide growth inhibitor from bovine mammary gland. J Biol Chem 262, 15137–15143
Chevalet C, Corpet F (1986) Statistical decision rules concerning synteny or independence between markers. Cytogenet Cell Genet 43, 132–139
Claffey KP, Herrera VL, Brecher P, Ruiz-Opazo N (1987) Cloning and tissue distribution of rat heart fatty acid-binding protein mRNA: identical forms in heart and skeletal muscle. Biochemistry 26, 7900–7904
Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th ed. (Essex, England: Longman Group), pp 1–19
Heuckeroth RO, Birkenmeier EH, Levin MS, Gordon JI (1987) Analysis of the tissue-specific expression, developmental regulation, and linkage relationships of a rodent gene encoding heart fatty acid binding protein. J Biol Chem 262, 9709–9717
Hovenier R, Kanis E, Van Asseldonk Th, Westerink NG (1992) Genetic parameters of pig meat quality traits in a halothane negative population. Livest Prod Sci 32, 309–321
Janss LLG, Van Arendonk JAM, Brascamp EW (1994) Identification of a single gene affecting intramuscular fat in Meishan crossbreds using Gibbs sampling. World Conference on Genetics Applied to Livestock Production, Guelph
Liu K, Robinson GW, Gouilleux F, Groner B, Henninghausen L (1995) Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue. Proc Natl Acad Sci USA 92, 8831–8835
MacDougald OA, Lane MD (1995) Transcriptional regulation of gene expression during adipocyte differentiation. Annu Rev Biochem 64, 345–373
Olson NE, Perry M, Schulz RA (1995) Regulation of muscle differentiation by the MEF-2 family of MADS box transcription factors (mini review). Dev Biol 172, 2–14
Paszek AA, Schook LB, Louis CF, Mickelson JR, Flickinger GH, Murtaugh J, Mendiola JR, Janzen MA, Beattie CW, Rohrer GA, Alexander LJ, Andersson L, Ellegren H, Johansson M, Mariani P, Marklund L, Hoyheim B, Davies W, Fredholm M, Archibald AL, Haley CS (1995) First international workshop on porcine chromosome 6. Anim Gen 26, 377–401 (Abstract of J. Gellin)
Paulussen RJA, Van der Logt CPE, Veerkamp JH (1988) Characterization and binding properties of fatty acid binding proteins from human, pig and rat heart. Arch Biochem Biophys 264, 533–545
Paulussen RJA, Van Moerkerk HTB, Veerkamp JH (1990) Immunological quantification of fatty acid binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues. Int J Biochem 22, 393–398
Peeters RA, Veerkamp JH, Geurts van Kessel A, Kanda T, Ono T (1991) Cloning of the cDNA encoding human skeletal muscle fatty acid binding protein, its peptide sequence and chromosomal localization. Biochem J 276, 203–207
Rettenberger G, Klett C, Zechner U, Kunz J, Vogel W, Hameister H (1995) Visualization of the conservation between pigs and humans by heter-ologous chromosomal painting. Genomics 26, 372–378
Rettenberger G, Bruch J, Fries R, Archibald AL, Hameister H (1996) Assignment of 19 porcine type I loci by somatic cell hybrid analysis detects new regions of conserved synteny between human and pig. Mamm Genome 7, 275–279
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. (Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory)
Specht B, Bartetzko N, Hohoff C, Kuhl H, Franke R, Börchers T, Spener F. (1996) Mammary derived growth inhibitor is not a distinct protein but a mix of heart-type and adipocyte-type fatty acid-binding protein. J Biol Chem 271, 19943–19949
Spener F, Unterberg C, Börchers T, Grosse R (1990) Characteristics of fatty acid-binding proteins and their relation to mammary-derived growth inhibitor. Mol Cell Biochem 98, 57–68
Treuner M, Kozak CA, Gallahan D, Grosse R, Muller T (1994) Cloning and characterization of the mouse gene encoding mammary-derived growth inhibitor/heart fatty acid-binding protein. Gene 147, 237–242
Troxler RF, Offner GD, Jian JW, Wu BL, Skare JC, Milunsky A, Wyandt HE (1993) Localization of the gene for human heart fatty acid binding protein to chromosome Ip32-lp33. Hum Genet 92, 536–566
Veerkamp JH, Maatman RGHJ (1995) Cytoplasmic fatty acid binding proteins: their structure and genes. Progr Lipid Res 34, 17–52
Veerkamp JH, Van Kuppevelt THMSM, Maatman RGHJ, Prinsen CFM (1993) Structural and functional aspects of cytosolic fatty acid binding proteins. Prostaglandins Leukot Essent Fatty Acids 49, 887–906
Watson CJ, Gordon KE, Robertson M, Clark AJ (1991) Interaction of DNA-binding proteins with a milk protein gene promoter in vitro: identification of a mammary gland-specific factor. Nucleic Acids Res 19, 6603–6610
Wood JD, Enser M, Moncrieff CB, Kempster AJ (1988) Effects of carcass fatness and sex on the composition and quality of pigmeat. 34th International Congress of Meat Science and Technology. August 29-September 2, 1988, Brisbane, Australia, pp 562–564
Yerle M, Lahib-Mansais Y, Mellink C, Goureau A, Pinton P, Echard G, Gellin J, Zijlstra C, De Haan N, Bosma AA, Chowdhary B, Gu F, Gustavsson I, Thomsen PD, Christensen K, Rettenberger G, Hameister H, Schmitz A, Chaput B, Frelat G (1995) The PIGMaP consortium cytogenetic map of the domestic pig (Sus scrofa domestica). Mamm Genome 9, 176–186
Zijlstra C, Bosma AA, de Haan NA (1994) Comparative study of pigrodent somatic cell hybrids. Anim Genet 25, 319–327
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to the EMBL database and have been assigned the accession numbers X98555, X98556, X98557, and X98558.
Rights and permissions
About this article
Cite this article
Gerbens, F., Rettenberger, G., Lenstra, J.A. et al. Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene. Mammalian Genome 8, 328–332 (1997). https://doi.org/10.1007/s003359900433
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s003359900433