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Effect of high hydrostatic pressure on hydration and activity of ribozymes

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Abstract

Formation and stabilization of RNA structure in the cell depends on its interaction with solvent and metal ions. High hydrostatic pressure (HHP) is a convenient tool in an analysis of the role of small molecules in the structure stabilization of biological macromolecules. Analysis of HHP effect and various concentrations of ions showed that water induce formation of the active ribozyme structure. So, it is clear that water is the driving force of conformational changes of nucleic acid.

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Abbreviations

HHP:

High hydrostatic pressure

AP:

Atmospheric pressure

HH:

Hammerhead

FTIR:

Fourier transform infrared spectroscopy

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12/14, 61-704, Poznan, Poland

    Małgorzata Giel-Pietraszuk, Agnieszka Fedoruk-Wyszomirska & Jan Barciszewski

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  1. Małgorzata Giel-Pietraszuk
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  2. Agnieszka Fedoruk-Wyszomirska
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  3. Jan Barciszewski
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Correspondence to Jan Barciszewski.

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Giel-Pietraszuk, M., Fedoruk-Wyszomirska, A. & Barciszewski, J. Effect of high hydrostatic pressure on hydration and activity of ribozymes. Mol Biol Rep 37, 3713–3719 (2010). https://doi.org/10.1007/s11033-010-0024-3

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