Skip to main content
SpringerLink
Log in

Associations of polymorphism within the GHSR gene with growth traits in Nanyang cattle

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

GH secretagogue receptor (ghrelin receptor, GHSR) is known to be involved in the control of GH release by mediating the strong stimulatory effect of the endogenous ligand, ghrelin, on GH secretion. Associations between the GHSR gene polymorphism and the growth traits were revealed in Nanyang cattle. The mutations at nt456(G > A) and nt667(C > T) were complete linkage and located in exon 1 of the coding region of the GHSR gene. Least squares analysis revealed a significant statistical effect (P < 0.05) of the GHSR gene different genotypes on body weight and average daily gain at 6 months of age in Nanyang cattle. Individuals with GHSR-MM genotype showed higher body weight and average daily gain than individuals with GHSR-MN genotype.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Lande R (1981) The minimum number of genes contributing to quantitative variation between and within populations. Genetics 99:541–553

    CAS  PubMed  Google Scholar 

  2. Wren AM, Seal LJ, Cohen MA, Brynes AE, Frost GS, Murphy KG, Dhillo WS, Ghatei MA, Bloom SR (2001) Ghrelin enhances appetite and increases food intake in humans. J Clin Endocrinol Metab 86(12):5992–5995. doi:10.1210/jc.86.12.5992

    Article  CAS  PubMed  Google Scholar 

  3. Wren AM, Small CJ, Abbott CR, Dhillo WS, Seal LJ, Cohen MA, Batterham RL, Taheri S, Stanley SA, Ghatei MA, Bloom SR (2001) Ghrelin causes hyperphagia and obesity in rats. Diabetes 50:2540–2547. doi:10.2337/diabetes.50.11.2540

    Article  CAS  PubMed  Google Scholar 

  4. Broglio F, Arvat E, Benso A, Gottero C, Muccioli G, Papotti M, Lely AJ, Deghenghi R, Ghigo E (2001) Ghrelin, a natural GH secretagogue produced by the stomach, induces hyperglycemia and reduces insulin secretion in humans. J Clin Endocrinol Metab 86:5083–5086. doi:10.1210/jc.86.10.5083

    Article  CAS  PubMed  Google Scholar 

  5. Tschop M, Weyer C, Tataranni PA, Devanarayan V, Ravussin E, Heiman ML (2001) Circulating ghrelin levels are decreased in human obesity. Diabetes 50:707–709. doi:10.2337/diabetes.50.4.707

    Article  CAS  PubMed  Google Scholar 

  6. Cummings DE, Weigle DS, Frayo RS, Breen PA, Ma MK, Dellinger EP, Purnell JQ (2002) Plasma ghrelin levels after diet-induced weight loss or gastric bypass surgery. N Engl J Med 346:1623–1630. doi:10.1056/NEJMoa012908

    Article  PubMed  Google Scholar 

  7. Marzullo P, Verti B, Savia G, Walker GE, Guzzaloni G, Tagliaferri M, Di Blasio A, Liuzzi A (2004) The relationship between active ghrelin levels and human obesity involves alterations in resting energy expenditure. J Clin Endocrinol Metab 89:936–939. doi:10.1210/jc.2003-031328

    Article  CAS  PubMed  Google Scholar 

  8. Zigman JM, Nakano Y, Coppari R, Balthasar N, Marcus JN, Lee CE, Jones JE, Deysher AE, Waxman AR, White RD, Williams TD, Lachey JL, Seeley RJ, Lowell BB, Elmquist JK (2005) Mice lacking ghrelin receptors resist the development of diet-induced obesity. J Clin Invest 115:3564–3572. doi:10.1172/JCI26002

    Article  CAS  PubMed  Google Scholar 

  9. Smith RG (2005) Development of growth hormone secretagogues. Endocr Rev 26(3):346–360. doi:10.1210/er.2004-0019

    Article  CAS  PubMed  Google Scholar 

  10. Wang HJ, Geller F, Dempfle A, Schäuble N, Friedel S, Lichtner P, Fontenla-Horro F, Wudy S, Hagemann S, Gortner L, Huse K, Remschmidt H, Bettecken T, Meitinger T, Schäfer H, Hebebrand J, Hinney A (2004) Ghrelin receptor gene: identification of several sequence variants in extremely obese children and adolescents, healthy normal-weight and underweight students, and children with short normal stature. J Clin Endocrinol Metab 89:157–162. doi:10.1210/jc.2003-031395

    Article  CAS  PubMed  Google Scholar 

  11. Miyasakal K, Hosoyal H, Sekimel A, Ohtal M, Amono H, Matsushita S, Suzuki K, Higuchi S, Funakoshi A (2006) Association of ghrelin receptor gene polymorphism with bulimia nervosa in a Japanese population. J Neural Transm 113:1279–1285. doi:10.1007/s00702-005-0393-2

    Article  Google Scholar 

  12. Pantel J, Legendre M, Cabrol S, Hilal L, Hajaji Y, Morisset S, Nivot S, Vie-Luton M, Grouselle D, Kerdanet MD, Kadiri A, Epelbaum J, Bouc YL, Amselem S (2006) Loss of constitutive activity of the growth hormone secretagogue receptor in familial short stature. J Clin Invest 116:760–768. doi:10.1172/JCI25303

    Article  CAS  PubMed  Google Scholar 

  13. Zhang B, Chen H, Yk Guo, Zhang LZ, Hua LS, Zhao M, Lan XY, Lei CZ (2007) Five novel single nucleotide polymorphisms (SNPs) of the ghrelin receptor (GHSR) gene in cattle. Arch. Tierz. Dummerstorf 6:630–631

    Google Scholar 

  14. Sambrook J, Russell DW (2002) Translated by huang pei tang. Molecular cloning a laboratory manual, 3rd edn. Science Press, Beijing

    Google Scholar 

  15. Tschop M, Smiley DL, Heiman ML (2000) Ghrelin induces adiposity in rodents. Nature 407:908–913. doi:10.1038/35038090

    Article  CAS  PubMed  Google Scholar 

  16. Nakazato M, Murakami N, Date Y, Kojima M, Matsuo H, Kangawa K, Matsukura S (2001) A role for ghrelin in the central regulation of feeding. Nature 409:194–198. doi:10.1038/35051587

    Article  CAS  PubMed  Google Scholar 

  17. Cummings DE, Schwartz MW (2003) Genetics and pathophysiology of human obesity. Annu Rev Med 54:453–471. doi:10.1146/annurev.med.54.101601.152403

    Article  CAS  PubMed  Google Scholar 

  18. Holst B, Cygankiewicz A, Jensen TH, Ankersen M, Schwartz TW (2003) High constitutive signaling of the ghrelin receptor–identification of a potent inverse agonist. Mol Endocrinol 17:2201–2210. doi:10.1210/me.2003-0069

    Article  CAS  PubMed  Google Scholar 

  19. Casanueva FF, Dieguez C (2002) Ghrelin: the link connecting growth with metabolism and energy homeostasis. Rev Endocr Metab Disord 3:325–338. doi:10.1023/A:1020901624103

    Article  CAS  PubMed  Google Scholar 

  20. Howard AD, Feighner SD, Cully DF, Arena JP, Liberator PA, Rosenblum CI, Hamelin M, Hreniuk DL, Palyha OC, Anderson J, Paress PS, Diaz C, Chou M, Liu KK, McKee KK, Pong S, Chaung LY, Elbrecht A, Dashkevicz M, Heavens R, Rigby M, Sirinathsinghji DJ, Dean DC, Melillo DG, Patchett AA, Nargund R, Griffin PR, DeMartino JA, Gupta SK, Schaeffer JM, Smith RG (1996) A receptor in pituitary and hypothalamus that functions in growth hormone release. Science 273:974–977. doi:10.1126/science.273.5277.974

    Article  CAS  PubMed  Google Scholar 

  21. Baessler A, Kwitek AE, Fischer M, Koehler M, Reinhard W, Erdmann J, Riegger G, Doering A, Schunkert H, Hengstenberg C (2006) Association of the ghrelin receptor gene region with left ventricular hypertrophy in the general population. Hypertension 47:920–927. doi:10.1161/01.HYP.0000215180.32274.c8

    Article  CAS  PubMed  Google Scholar 

  22. Baessler A, Hasinoff MJ, Fischer M, Reinhard W, Sonnenberg GE, Olivier M, Erdmann J, Schunkert H, Doering A, Jacob HJ, Comuzzie AG, Kissebah AH, Kwitek AE (2005) Genetic linkage and association of the growth hormone secretagogue receptor (ghrelin receptor) gene in human obesity. Diabetes 54:259–267. doi:10.2337/diabetes.54.1.259

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by the National 863 Program of China (No. 2006AA10Z197), National Natural Science Foundation of China (No. 30771544), National Key Technology R&D Program (No. 2006BAD01A10-5), Innovative Foundation of Outstanding Talent from Henan Province (No. 0521001900), Sustaining Program for Topnotch Persons of Northwest A&F University (No. 01140101), and Natural Science Foundation of Xuzhou Normal University and Talent Foundation of Northwest A&F University, Basic and Foreland Technology Study Program of Henna Province (No. 072300430160), “13115” Sci-Tech Innovation Program of Shaanxi Province (2008ZDKG-11).

Author information

Authors and Affiliations

  1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Bao Zhang, Hong Chen, Yikun Guo, Liangzhi Zhang, Miao Zhao, Xianyong Lan, Chuanying Pan, Shenrong Hu & Chuzhao Lei

  2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, 221116, Xuzhou, Jiangsu, China

    Hong Chen & Chunlei Zhang

  3. Research Center of Cattle Engineering Technology in Henan, 450003, Zhengzhou, Henan, Peoples Republic of China

    Juqiang Wang

Authors
  1. Bao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yikun Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liangzhi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Miao Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xianyong Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chunlei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Chuanying Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shenrong Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Juqiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Chuzhao Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hong Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, B., Chen, H., Guo, Y. et al. Associations of polymorphism within the GHSR gene with growth traits in Nanyang cattle. Mol Biol Rep 36, 2259–2263 (2009). https://doi.org/10.1007/s11033-008-9442-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-008-9442-x

Keywords

Search

Navigation