Abstract
Panicle structure is one of the most important agronomic traits directly related to rice yield. This study identified a rice mutant basal primary branch 1 (bpb1), which exhibited a phenotype of reduced panicle length and arrested basal primary branch development. In addition, lignin content was found to be increased while cellulose content was decreased in bpb1 young panicles. Map-based cloning methods characterized the gene BPB1, which encodes a peptide transporter (PTR) family transporter. Phylogenetic tree analysis showed that the BPB1 family is highly conserved in plants, especially the PTR2 domain. It is worth noting that BPB1 is divided into two categories based on monocotyledonous and dicotyledonous plants. Transcriptome analysis showed that BPB1 mutation can promote lignin synthesis and inhibit cellulose synthesis, starch and sucrose metabolism, cell cycle, expression of various plant hormones, and some star genes, thereby inhibiting rice panicle length, resulting in basal primary branch development stagnant phenotypes. In this study, BPB1 provides new insights into the molecular mechanism of rice panicle structure regulation by BPB1 by regulating lignin and cellulose content and several transcriptional metabolic pathways.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors extend their appreciation to the support from Collaborative Innovation Center of Henan Grain Crops and Henan Key Laboratory of Rice Biology, Henan Agricultural University.
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This work was supported by the Project Tackling of Key Scientific and Technical Problems of Henan (212102110284) and Key Scientific Research Projects of Colleges and Universities in Henan Province (23A210027), received grants from the Natural Science Foundation of Henan (212300410357) and the Special Fund for Henan Agriculture Research System (HARS-22–03-G3), and sponsored by Natural Science Foundation of Henan (232300421156).
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Fei Li conducted transcriptome analysis, generated the figures and tables, wrote the original draft, review, and editing. Ke Wang conducted most of the experiments, including genetic effect validation and fine mapping. Xiaohua Zhang and Peijie Han performed rice pericarp sampling, RNA extraction and qRT-PCR. Ye Liu prepared Fig. 5. Jing Zhang, Ting Peng, Junzhou Li, and Yafan Zhao review and editing. Hongzheng Sun reviewed, edited, and revised the manuscript. Yanxiu Du designed the experiments, supervision, funding acquisition, resources, review, and editing. All authors discussed and commented on the manuscript.
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Li, F., Wang, K., Zhang, X. et al. BPB1 regulates rice (Oryza sative L.) panicle length and panicle branch development by promoting lignin and inhibiting cellulose accumulation. Mol Breeding 43, 41 (2023). https://doi.org/10.1007/s11032-023-01389-x
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DOI: https://doi.org/10.1007/s11032-023-01389-x