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Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice

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Abstract

Leaf morphology is an important agronomic trait in rice breeding. We isolated three allelic mutants of NARROW AND ROLLED LEAF 1 (nrl1) which showed phenotypes of reduced leaf width and semi-rolled leaves and different degrees of dwarfism. Microscopic analysis indicated that the nrl1-1 mutant had fewer longitudinal veins and smaller adaxial bulliform cells compared with the wild-type. The NRL1 gene was mapped to the chromosome 12 and encodes the cellulose synthase-like protein D4 (OsCslD4). Sequence analyses revealed single base substitutions in the three allelic mutants. Genetic complementation and over-expression of the OsCslD4 gene confirmed the identity of NRL1. The gene was expressed in all tested organs of rice at the heading stage and expression level was higher in vigorously growing organs, such as roots, sheaths and panicles than in elsewhere. In the mutant leaves, however, the expression level was lower than that in the wild-type. We conclude that OsCslD4 encoded by NRL1 plays a critical role in leaf morphogenesis and vegetative development in rice.

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Acknowledgments

We thank Dr. Bin Han (National Center for Gene Research and Institute of Plant Physiology and Ecology, Chinese Academy of Sciences) for providing BAC clones, Dr. Sheng Teng (Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences) for assisting in leaf sectioning, and Honglan Yan for photography. This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 30710103903, 30870145 and 30770127), China National Basic Research Priorities (Program 973), the Zhejiang Province Key Sci-Teach Project (2007c22018), Zhejiang Natural Science Foundation (Y307108).

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  1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, 359 Tiyuchang Road, 310006, Hangzhou, China

    Jiang Hu, Li Zhu, Dali Zeng, Zhenyu Gao, Longbiao Guo, Yunxia Fang, Guangheng Zhang, Guojun Dong, Meixian Yan, Jian Liu & Qian Qian

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  1. Jiang Hu
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  2. Li Zhu
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Correspondence to Qian Qian.

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Jiang Hu and Li Zhu have contributed equally to this work.

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Hu, J., Zhu, L., Zeng, D. et al. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Mol Biol 73, 283–292 (2010). https://doi.org/10.1007/s11103-010-9614-7

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