Abstract
Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a relative of wheat, carries desirable genes associated with high yield, disease resistance, and stress resistance, which is an important resource for wheat genetic improvement. The long arm of A. cristatum chromosome 2P carries favorable genes conferring powdery mildew and leaf rust resistance, and two wheat–A. cristatum 2P translocation lines, 2PT3 and 2PT5, with a large segment of 2P chromatin were obtained. In this study, 2PT3 and 2PT5 translocation lines with powdery mildew and leaf rust resistance genes were used to induce translocations of different chromosomal sizes via ionizing radiation. According to cytological characterization, 10 of those plants were new wheat–A. cristatum 2P small-chromosome segment translocation lines with reduced 2P chromatin, and 6 plants represented introgression lines without visible 2P chromosomal fragments. Moreover, four lines were resistant to both powdery mildew and leaf rust, while two lines were resistant only to leaf rust.
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This research was supported by the National Key Research and Development Program of China (2016YFD0100102).
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WHL and LHL designed the research. SRX and XJJ performed the research. WHL and LHL created the translocation lines. SLS, HMH, JPZ, SHZ, XMY, and XQL participated in the preparation of the reagents and materials used in this study. SRX wrote the manuscript. All authors read and approved the final manuscript.
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Xu, S., Ji, X., Sun, S. et al. Production of new wheat–A. cristatum translocation lines with modified chromosome 2P coding for powdery mildew and leaf rust resistance. Mol Breeding 42, 14 (2022). https://doi.org/10.1007/s11032-022-01286-9
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DOI: https://doi.org/10.1007/s11032-022-01286-9