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Production and characterization of a disomic 1M/1D Triticum aestivum-Aegilops comosa substitution line

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Abstract

PI 554419, formerly designated as Ae. uniaristata, showed significant difference with other Ae. uniaristata and Ae. comosa accessions in morphological traits at the seedling stage and its leaf color, length, and width behaved as an intermediate type. In this study, we reclassified PI 554419 as Ae. comosa subsp. comosa by comparing the fluorescence in situ hybridization (FISH) signals and the patterns of PCR-based landmark unique gene (PLUG) markers and conserved orthologous set (COS) markers of PI 554419 with other Ae. uniaristata and Ae. comosa accessions as well as the taxonomic character of spike morphology. A disomic 1M/1D substitution line NB 4-8-5-9 derived from PI 554419 was identified from a distant hybridization of Ae. comosa with common wheat (STM 10/CSph1b//CM 39///13 P2-6) by the molecular cytological method. Furthermore, the agronomic and seed morphological traits, as well as the flour processing quality properties of NB 4-8-5-9, were compared with those of its three common wheat parents in two different locations during the 2017–2018 growing seasons. The agronomical traits of NB 4-8-5-9 were similar to or even better than its parents. The seed size-related traits of NB 4-8-5-9 were better than those of all three parents, and the 1000-grain weight and grain width were close to those of Chuanmai 39 (CM 39) and 13 P2-6 and larger than those of CSph1b. The processing quality properties of NB 4-8-5-9 were more similar to those of 13 P2-6 and CSph1b but less similar to those of CM 39. The 1M/1D substitution line NB 4-8-5-9 could further be used for developing translocation lines with 1M segment.

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Data availability

The seeds in the current study are available from the corresponding author upon request.

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Acknowledgements

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This study is supported by the Sichuan Science and Technology Program (No. 2018HH0130 and No. 2018HH0113), the Natural National Science Foundation of China (31771783, U1403185), the Ministry of Science and Technology of China (2016YFD0100502, 2017YFD0100903), and the Key Research and Development Program of Sichuan Province (2018NZDZX0002).

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  1. Liangzhu Kang and Shoufen Dai contributed equally to this work.

Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China

    Liangzhu Kang, Shoufen Dai, Zhongping Song, Qin Xiang, Yuanyuan Zuo, Tingyu Bao, Guoyue Chen, Yuming Wei, Youliang Zheng, Gang Liu, Jian Li & Zehong Yan

  2. State Key laboratory of Crop Gene Exploration and Utilization in Southwest China, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China

    Shoufen Dai, Guoyue Chen, Yuming Wei, Youliang Zheng & Zehong Yan

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  1. Liangzhu Kang
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  12. Zehong Yan
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Contributions

KL Zhu wrote the paper. KL Zhu, SF Dai, ZP Song, Q Xiang, YY Zuo, and TY Bao conducted experiments and data analysis. Gang L and Jian L conduct field experiment. GY Chen, YM Wei, and YL Zhang give some suggestion. ZH Yan conceived and designed the study.

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Correspondence to Zehong Yan.

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Kang, L., Dai, S., Song, Z. et al. Production and characterization of a disomic 1M/1D Triticum aestivum-Aegilops comosa substitution line. Mol Breeding 41, 16 (2021). https://doi.org/10.1007/s11032-021-01207-2

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