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Construction of chromosome segment substitution lines of Chinese cabbage (Brassica rapa L. ssp. pekinensis) in the background of RcBr (B. rapa L. ssp. dichotoma) and characterization of segments representing the bolting trait

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Abstract

Chromosome segment substitution lines (CSSLs) are useful tools for precise mapping of quantitative trait loci (QTLs) and evaluation of gene actions or interactions in theoretical studies. To facilitate the genetic analysis of complex traits and use of marker-assisted breeding in Brassica rapa, we developed a set of CSSLs using the extremely early-flowering inbred line RcBr as the recipient and extremely late-bolting Chinese cabbage variety 08A061 as the donor parent. The CSSL population consisted of 120 lines containing a total of 275 substituted segments, of which 47 lines contained only one substituted segment. The total length of the substituted segments spanned 2489.75 cM with an average length of 9.05 cM, representing 91.0% of the B. rapa genome and each line consisting of an average 98.1% of the recurrent parent genome. A total of 15 putative additive QTLs and 10 pairwised epistatic QTLs for three bolting indices were subsequently detected by QTL IciMapping 3.2 under two growth environments. Phenotypic variation explained by the additive QTLs, with the total variation ranging from 3.31 to 31.29% and 10.04 to 75.83%, respectively. Overall, the epistatic QTLs explained only a small amount of the phenotypic variation. The CSSLs and analyses presented here will help enhance our understanding of the genetics of bolting in B. rapa, thereby facilitating future molecular breeding programs.

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Funding

This work was supported by the National Natural Science Foundation of China (NSFC) [grant number 31772296] and the Provincial Natural Science Foundation of Liaoning, China [grant number 20170540795].

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Authors and Affiliations

  1. College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China

    Yugang Wang, Xiaoshu Wang, Xian Wang, Qinan Zhao, Xixi Lv & Hui Feng

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  1. Yugang Wang
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  2. Xiaoshu Wang
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  3. Xian Wang
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  4. Qinan Zhao
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  5. Xixi Lv
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  6. Hui Feng
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Correspondence to Yugang Wang or Hui Feng.

Electronic supplementary material

Fig. S1

Lengths of the substituted chromosome segments in the 120 CSSLs. (GIF 85 kb)

High resolution image (TIFF 1892 kb)

Fig. S2

Frequency distribution in the CSSL population of the three bolting indices under the two test environments. (GIF 47 kb)

High resolution image (TIFF 40480 kb)

Fig. S3

Chromosomal positions of additive QTL in the CSSL and RIL populations. QTL for the three bolting indices (BI, DE, and FT) are shown in red, blue, and green, respectively. QTL identified in the CSSL and RIL populations are presented by solid and dotted lines, respectively. (GIF 3458 kb)

High resolution image (TIFF 2703 kb)

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Wang, Y., Wang, X., Wang, X. et al. Construction of chromosome segment substitution lines of Chinese cabbage (Brassica rapa L. ssp. pekinensis) in the background of RcBr (B. rapa L. ssp. dichotoma) and characterization of segments representing the bolting trait. Mol Breeding 38, 35 (2018). https://doi.org/10.1007/s11032-018-0794-1

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