Abstract
Protein content (PC) in rice endosperm plays an important role in determining rice grain quality. However, the genetic mechanism underlying grain PC remains unclear. In order to better understand the genetic basis of this trait, a chromosome segment substitution line (CSSL) population derived from the cross of Sasanishiki/Habataki was employed for genetic analysis and gene mapping. In three environments, seven quantitative trait loci in total were identified, of which only qPC-1 was repeatedly detected across three environments, and qPC-10 was identified in two environments; the other five QTLs were detected in one environment. In order to fine-map qPC-1, a CSSL with low PC, SL402, harboring qPC-1, was crossed with Sasanishiki to develop F2 and F3 segregation populations. qPC-1 was finally delimited to a 41-kb DNA region on chromosome 1. Storage protein component analysis indicated that the allele from Habataki on qPC-1 can significantly decrease the glutelin content, consequently leading to the decrease in PC. These results provide an important aid for map-based cloning of qPC-1, and the markers linked to qPC-1 could be applied to rice quality improvement.
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Acknowledgments
We thank the Rice Genome Project of the National Institute of Agrobiological Sciences of Japan for kindly providing the CSSL population. This study was supported financially by the National Natural Science Foundation (31371233), the Natural Science Foundation of Jiangsu Province (BK2012684, 14KJA210002), the project of Six Talent Peaks in Jiangsu Province (2012-NY-027), the research and innovation program of postgraduate in Jiangsu province (CXLX13_914), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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Yihao Yang and Min Guo have equally contributed equally to this work.
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Yang, Y., Guo, M., Li, R. et al. Identification of quantitative trait loci responsible for rice grain protein content using chromosome segment substitution lines and fine mapping of qPC-1 in rice (Oryza sativa L.). Mol Breeding 35, 130 (2015). https://doi.org/10.1007/s11032-015-0328-z
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DOI: https://doi.org/10.1007/s11032-015-0328-z