Abstract
Protein content (PC) and protein index (PI) play important roles in determining nutritional quality in rice (Oryza sativa L.). We used 71 lines derived from “Asominori/IR24” to analyze the developmental behavior of PC and PI through unconditional and conditional QTL mapping methods. In all, 10 unconditional QTLs and 6 conditional QTLs for PC, and 11 unconditional QTLs and 9 conditional QTLs for PI, were identified at four stages of grain filling. More were identified in the first three stages than at the final stage. Temporal patterns of gene expression for PC and PI differed over time, with several QTLs being expressed across two or three stages but many being expressed at only one stage. Some of these QTLs were closely linked with maturity QTLs reported previously. Many QTLs for PC and PI were co-localized, supporting the significant correlation found between PC and PI. Our results suggest that dynamic QTL mapping might be a valid means for revealing more genetic information about protein accumulations during seed development.
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Abbreviations
- DAF:
-
Days after flowering
- PC:
-
Protein content
- PI:
-
Protein index
- RFLP:
-
Restriction fragment length polymorphism
- RIL:
-
Recombinant inbred line
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Acknowledgements
This research was supported by grants from the National Key Transformation Program (2008ZX01001-006), the National Natural Science Foundation of China (30971759), the Jiangsu Cultivar Development Program (BE2008354 and BE2009301-3), the National Science and Technology Supporting Program, and the earmarked fund for a Modern Agro-industry Technology Research System.
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Zheng, L., Zhang, W., Chen, X. et al. Dynamic QTL Analysis of Rice Protein Content and Protein Index Using Recombinant Inbred Lines. J. Plant Biol. 54, 321–328 (2011). https://doi.org/10.1007/s12374-011-9170-y
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DOI: https://doi.org/10.1007/s12374-011-9170-y