Abstract
The spotted stem borer (SSB) Chilo sacchariphagus is a major pest of sugarcane, causing substantial losses in cane weight and in sucrose yield. SSB resistance is an important trait to be taken into account for sugarcane breeding programs. In order to analyse the genetic basis of the resistance to SSB, we undertook a quantitative trait allele (QTA) mapping study based on a population of 147 progenies derived from the selfing of the resistant modern cultivar R570. The experimental population was evaluated in a replicated trial for borer damage under natural infestation in two successive crop cycles. A single-factor analysis using 1,405 polymorphic markers was performed to detect marker–trait associations. Statistical thresholds based on permutation tests designed to control type I errors at a low level allowed the detection of nine QTAs whose individual size ranged between 6 and 10% of the total variation. These nine QTAs are distributed over five of the eight homeology groups of the polyploid R570 genome. Two QTAs were found to co-localize with two typical resistance gene analog clusters. Overall, eight QTAs explain altogether 42% of the total phenotypic variance.
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References
Aitken K, Hermann S, Karno K, Bonnett G, McIntyre L, Jackson P (2008) Genetic control of yield related stalk traits in sugarcane. Theor Appl Genet 117:1191–1203
Bezuidenhout CN, Goebel R, Hull PJ, Hull PJ, Schulze RE, Maharaj M (2008) Assessing the potential threat of Chilo sacchariphagus (Lepidoptera: Crambidae) as a pest in South Africa and Swaziland: realistic scenarios based on climatic indices. Afr Entomol 16:86–90. doi:10.4001/1021-3589-16.1.86
Butterfield M, Rutherford R, Carson D, Huckett B (2004) Application of gene discovery to varietal improvement in sugarcane. S Afr J Bot 70:167–172
Churchill GA, Doerge RW (1994) Empirical Threshold Values for Quantitative Trait Mapping. Genetics 138:963–971
Da Silva JA, White WH, Setamou M, Solis-Garcia N (2005) A molecular approach to breeding for stemborer resistance in sugarcane. In: Hogarth DM (ed) Proceedings of the XXV Congress of the international society of sugar cane technologists, January 30–February 4, Guatemala City, Guatemala, 2005. ISSCT, pp 487–491
Daugrois JH, Grivet L, Roques D, Hoarau JY, Lombard H, Glaszmann JC, D’Hont A (1996) A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar ‘R570’. Theor Appl Genet 92:1059–1064
Gallais A (1990) Théorie de la sélection en amélioration des plantes. Masson, Paris
Goebel R (1999) Caractéristiques biotiques du foreur de la canne à sucre Chilo sacchariphagus (Bojer, 1856) (Lepidoptera : Pyralidae) à l’île de la Réunion. Facteurs de régulation de ses populations et conséquences pour la lutte contre ce ravageur. Université Paul Sabatier, Toulouse
Goebel R, Fernandez E, Tibère R, Alauzet C (1999) Dégâts et pertes de rendement sur la canne à sucre dus au foreur Chilo sacchariphagus (Bojer) à l’île de la Réunion (Lep.: Pyralidae). Ann Soc Entomol France 35(Supp):476–481
Grivet L, D’Hont A, Roques D, Feldmann P, Lanaud C, Glaszmann JC (1996) RFLP mapping in cultivated sugarcane (Saccharum spp.): genome organization in a highly polyploid and aneuploid interspecific hybrid. Genetics 142:987–1000
Hoarau JY, Offmann B, D’Hont A, Risterucci AM, Roques D, Glaszmann JC, Grivet L (2001) Genetic dissection of a modern sugarcane cultivar (Saccharum spp.). I. Genome mapping with AFLP markers. TAG Theor Appl Genet 103:84–97
Hoarau JY, Grivet L, Offmann B, Raboin LM, Diorflar JP, Payet J, Hellmann M, D’Hont A, Glaszmann JC (2002) Genetic dissection of a modern sugarcane cultivar (Saccharum spp.).II. Detection of QTLs for yield components. Theor Appl Genet 105:1027–1037
Holland JB (2006) Estimating genotypic correlations and their standard errors using multivariate restricted likelihood estimation with SAS proc mixed. Crop Sci 46:642–654
Hulbert SH, Webb CA, Smith SM, Sun Q (2001) Resistance gene complexes: evolution and utilization. Ann Rev Phytopathol 39:285–312. doi:10.1146/annurev.phyto.39.1.285
Jannoo N, Grivet L, David J, D’Hont A, Glaszmann JC (2004) Differential chromosome pairing affinities at meiosis in polyploidy sugarcane revealed by molecular markers. Heredity 93:460–467
Lander E, Kruglyak L (1995) Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nature Genet 11:241–247
Littel RC, Milliken GA, Stroup WW, Wolfinger RD, Schabenberger O (2006) SAS system for mixed models, 2nd edn. SAS Institute Inc., Cary
Martin G, Brommonschenkel S, Chunwongse J, Frary A, Ganal M, Spivey R, Wu T, Earle E, Tanksley S (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436
McIntyre C, Casu R, Drenth J, Knight D, Whan V, Croft B, Jordan D, Manners J (2005) Resistance gene analogues in sugarcane and sorghum and their association with quantitative trait loci for rust resistance. Genome 48:391–400
McMullen MD, Simcox KD (1995) Genomic organization of disease and insect resistance genes in maize. Mol Plant-Microbe Interact 8:811–815
Metcalfe JR (1969) The estimation of loss caused by sugar cane moth borers. In: Williams JR, Metcalfe JR, Mungomery RW, Mathes R (eds) Pests of sugarcane. Elsevier Publishing Company, Amsterdam, pp 61–79
Ming R, Wang Y, Draye X, Moore P, Irvine J, Paterson A (2002) Molecular dissection of complex traits in autopolyploids: mapping QTLs affecting sugar yield and related traits in sugarcane. Theor Appl Genet 105:332–345
Nibouche S, Tibere R (2010) Mechanism of resistance to the spotted stalk borer, Chilo sacchariphagus, in the sugarcane cultivar R570. Entomol Exp Appl 135:308–314. doi:10.1111/j.1570-7458.2010.00996.x
Nibouche S, Tibère R (2008) Damage assessment for selection of resistance to the spotted stalk borer and genetic correlations for resistance and yield components in sugarcane. Plant Breed 127:38–42. doi:10.1111/j.1439-0523.2008.01391.x
Raboin L, Oliveira K, Lecunff L, Telismart H, Roques D, Butterfield M, Hoarau J, D’Hont A (2006) Genetic mapping in sugarcane, a high polyploid, using bi-parental progeny: identification of a gene controlling stalk colour and a new rust resistance gene. Theor and Appl Genet 112:1382–1391
Rossi M, Araujo PG, Paulet F, Garsmeur O, Dias VM, Chen H, Van Sluys MA, D’Hont A (2003) Genomic distribution and characterization of EST-derived resistance gene analogs (RGAs) in surgarcane. Mol Genet Genomics 269:406–419
Sakamoto K, Tada Y, Yokozeki Y, Akagi H, Hayashi N, Fujimura T, Ichikawa N (1999) Chemical induction of disease resistance in rice is correlated with the expression of a gene encoding a nucleotide binding site and leucine-rich repeats. Plant Mol Biol 40:847–855
SAS Institute (2004) SAS OnlineDoc® 9.1.3. SAS Institute Inc, Cary
Selvi A, Mukunthan N, Shanthi RM, Govindaraj P, Singaravelu B, Karthik Prabu T (2008) Assessment of genetic relationships and marker identification in sugarcane cultivars with different levels of top borer resistance. Sugar Tech 10:53–59
Acknowledgments
We thank R. Tibère and C. Lallemand for field work. The authors also wish to thank J. Dintinger for his critical review of this paper. This work was funded by the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), by the Conseil Régional de la Réunion and by the European Union: European Agricultural Guidance and Guarantee Fund (AEGGF).
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Nibouche, S., Raboin, L.M., Hoarau, JY. et al. Quantitative trait loci for sugarcane resistance to the spotted stem borer Chilo sacchariphagus . Mol Breeding 29, 129–135 (2012). https://doi.org/10.1007/s11032-010-9531-0
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DOI: https://doi.org/10.1007/s11032-010-9531-0