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Determination of the Metrological Characteristics of the Near-Field Scanning Optical Microscope in the Study of Biological Objects

  • MEDICAL AND BIOLOGICAL MEASUREMENTS
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Measurement Techniques Aims and scope

Methods of determining the basic metrological characteristics of a near-field scanning optical microscope, i.e., scale factor and spatial resolution, defined as the diameter of the optical probe, are proposed. The use of the proposed method for calibration of the near-field scanning optical microscope directly in the course of measurements of the parameters of biological objects is investigated.

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References

  1. M. Koopman et al., “Near-field scanning optical microscopy in liquid for high resolution single molecule detection on dendritic cells,” FEBS Lett., No. 573, 6–10 (2004).

    Google Scholar 

  2. J. Hwang et al., “Domains in cell plasma membranes investigated by near-field scanning optical microscopy,” J. Biophys., No. 74, 2184–2190 (1998).

    Google Scholar 

  3. T. Enderle et al., “Membrane specific mapping and colocalization of malarial and host skeletal proteins in the Plasmodium falciparum infected erythrocyte by dual-color near-field scanning optical microscopy,” PNAS, No. 94, 520–525 (1997).

  4. R. Dunn, “Near-field scanning optical microscopy,” Chem. Rev., No. 99, 2891–2897 (1999).

    Google Scholar 

  5. L. Reimer, Scanning Electron Microscopy, Springer-Verlag, Berlin (1985).

    Book  Google Scholar 

  6. J. Vigoureux, F. Depasse, and C. Girard, “Superresolution of near-field optical microscopy defined from properties of confined electromagnetic waves,” App. Opt., No. 31, 3036–3045 (1992).

    Google Scholar 

  7. H. Bethe, “Theory of diffraction by small holes,” Phys. Rev., No. 66, 163–182 (1944).

    Google Scholar 

  8. G. S. Zhdanov, M. N. Libenson, and G. A. Martsinovskij, Near-Field Optics [in Russian], GU ITMO, St. Petersburg (2007).

    Google Scholar 

  9. Sy-Hann Chen et al., “Convenient near-field optical measurement and analysis of polystyrene spheres,” Vacuum, No. 81, 129–132 (2006).

  10. Industrial Metrology: Surfaces and Roundness, Springer-Verlag, London (2002).

    Google Scholar 

  11. F. V. Bulygin and V. L. Lyaskovskii, “Investigation of methods for the measurement of the diameter of the electron probe of scanning electron microscopes by means of modern nanometric-range measures,” Metrologiya, No. 7, 34–43 (2010).

    Google Scholar 

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Authors and Affiliations

  1. All-Russia Research Institute of Optophysical Measurements (VNIIOFI), Moscow, Russia

    F. V. Bulygin & O. E. Dracheva

  2. Lomonosov Moscow State University, Moscow, Russia

    N. P. Kutuzov, V. L. Lyaskovskii, G. V. Maksimov & Yu. A. Nikolaev

Authors
  1. F. V. Bulygin
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  2. O. E. Dracheva
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  3. N. P. Kutuzov
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  4. V. L. Lyaskovskii
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  5. G. V. Maksimov
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  6. Yu. A. Nikolaev
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Corresponding author

Correspondence to O. E. Dracheva.

Additional information

Translated from Izmeritel’naya Tekhnika, No. 10, pp. 55–59, October, 2013.

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Bulygin, F.V., Dracheva, O.E., Kutuzov, N.P. et al. Determination of the Metrological Characteristics of the Near-Field Scanning Optical Microscope in the Study of Biological Objects. Meas Tech 56, 1173–1180 (2014). https://doi.org/10.1007/s11018-014-0351-1

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  • DOI: https://doi.org/10.1007/s11018-014-0351-1

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