Abstract
Oxidative stress (OS) is well established as a major event in Alzheimer’s disease (AD) pathology. One of the mostly-researched classes of antioxidants to manage with overwhelming OS include flavonoids. This study was aimed to investigate the protective effect of A. congolensis extract (HEEAC) on AlCl3-mediated AD-like OS and assess the contribution of its antioxidant flavonoid contents. Female Wistar (250–300 g) rats received orally 50 mg/Kg bw of AlCl3, followed one hour later by doses (150 or 300 mg/kg) of HEEAC or vitamin E at 100 mg/kg daily for eight consecutive weeks. OS related biomarkers were evaluated at the end of treatment. To assess the contribution of flavonoid contents to its activity, HEEAC was fractioned using solvent of varying polarities. Flavonoid-rich extracts obtained were tested for their antioxidant capacity. AlCl3 administration significantly lowered antioxidant enzymes (catalase, glutathione peroxidase) and aconitase levels, reduced total thiol and thiol protein levels and increased lipid peroxidation and protein oxidation levels in brain. When co-administrated with HEEAC at 150 mg/kg, all of these OS related biomarkers were significantly moderated. The efficacity of the extract was significantly higher than vitamin E. Flavonoid-rich fractions extracted mainly n-butanol fraction show strong antioxidant activity, which can be considered as the major antioxidant fraction of this plant. HEEAC protect brain cells against oxidative damage induced by AlCl3, specifically through the strong antioxidant property of its n-butanol flavonoid-rich fraction, which may be a promising agent for preventing oxidative damage in AD.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Ngoumen Ngassa Dany Joël, Ella Fils Armand, Ambamba Akamba Bruno Dupon and Nanhah Kamga Jules Vidal. The first draft of the manuscript was written by Ngoumen Ngassa Dany Joël, Mandob Enyegue Damaris, Fonkoua Martin, Ngondi Judith Laure and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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This study was approved by the Animal Ethics Committee of the Faculty of Sciences, University of Yaounde 1, Yaounde, Cameroon and in accordance with the principles of the “Guide to the Care and Use of Experimental Animals (Committee on Care and Use of Laboratory Animals, 1985)’’.
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Ngoumen, D.J.N., Mandob, D.E., Ella, F.A. et al. Flavonoid-enrich extract of Autranella congolensis (Sapotaceae) protects against aluminium chloride-mediated Alzheimer’s disease-like oxidative stress in rat through the antioxidant properties. Metab Brain Dis 38, 1025–1034 (2023). https://doi.org/10.1007/s11011-022-01142-x
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DOI: https://doi.org/10.1007/s11011-022-01142-x