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Effect of RNA-binding proteins on osteogenic differentiation of bone marrow mesenchymal stem cells

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Abstract

Tissue regeneration mediated by mesenchymal stem cells (MSCs) is an ideal way to repair bone defects. RNA-binding proteins (RBPs) can affect cell function through post-transcriptional regulation. Exploring the role of RBPs in the process of osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is helpful to find a key method to promote the osteogenic efficiency of BMSCs. By reviewing the literature, we obtained a differentially expressed mRNA dataset during the osteogenic differentiation of BMSCs and a human RBP dataset. A total of 82 differentially expressed RBPs in the osteogenic differentiation of BMSCs were screened by intersection of the two datasets. Functional analysis showed that the differentially expressed RBPs were mainly involved in RNA transcription, translation and degradation through the formation of spliceosomes and ribonucleoprotein complexes. The top 15 RBPs determined by degree score were FBL, NOP58, DDX10, RPL9, SNRPD3, NCL, IFIH1, RPL18A, NAT10, EXOSC5, ALYREF, PA2G4, EIF5B, SNRPD1 and EIF6. The results of this study demonstrate that the expression of many RBPs changed during osteogenic differentiation of BMSCs.

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All data generated or analysed during this study are included in this published article [and its Additional files].

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Funding

This work was supported by supported by the National Natural Science Foundation of China (Grant numbers 82170980 and 81771094), Young Scientist Program of Beijing Stomatological Hospital, Capital Medical University (NO. YSP202207).

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  1. Department of Prosthodontics, School of Stomatology, Beijing Stomatological Hospital, Capital Medical University, No. 4 Tiantan Xili, Beijing, 100050, China

    Bin Luo & Qingsong Jiang

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BL: conceived and designed this research, collected and analysed data, and wrote the manuscript. QJ: conceived and designed experiments and revised the manuscript. All authors have read and approved the manuscript.

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Luo, B., Jiang, Q. Effect of RNA-binding proteins on osteogenic differentiation of bone marrow mesenchymal stem cells. Mol Cell Biochem 479, 383–392 (2024). https://doi.org/10.1007/s11010-023-04742-y

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