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Silencing SATB1 inhibits proliferation of human osteosarcoma U2OS cells

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Abstract

It has been shown that over-expression of Special AT-rich binding protein 1 (SATB1) in breast cancer predicts a poor prognosis. This study was aimed at investigating the effects of silencing SATB1 on mesenchymal derived human osteosarcoma U2OS cells and the underlying mechanisms. The expressions of SATB1 and the related genes in the cells were detected by qRT-PCR and/or Western Blotting. SATB1 silencing was achieved by stable transfection with the vectors expressing small hairpin RNA versus SATB1. Cell proliferation was detected in a microplate reader with Cell Counting Kit-8 and the cell cycle was analyzed by flow cytometry using a cell cycle detection kit. The study found that SATB1 was particularly over-expressed in human osteosarcoma U2OS. Silencing SATB1 inhibited the proliferation of U2OS. It was found that inhibition of cell proliferation resulted from cell cycle arrest due to down-regulated expression of CFGF and JunB. The over-expression of SATB1 is responsible for abnormal proliferation of mesenchymal derived human Osteosatcoma U2OS cells, indicating that silencing SATB1 expression in the cells might be developed as an efficient osteosarcoma therapy. CTGF and JunB were involved in SATB1-mediated proliferation of U2OS cells.

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Acknowledgments

This study was supported in part by the Platform Fund for The Key Laboratory of Pathobiology, Ministry of Education, Jilin University (No. 450060481302) and Jilin University Initiation Fund (No. 450091105188) for “The Tang Aoqing Distinguished Professor.” We would like to express our great appreciation to Dr. Xuejin Su, for his excellent technical assistance and to Professor F. William Orr from the University of Manitoba in Canada for his great help in revising the manuscript.

Conflict of interest

The authors declare that they have no conflicting or competing interests.

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Authors and Affiliations

  1. Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, People’s Republic of China

    Haiying Zhang, Shuang Li, Yang Wang, Yulin Li, Yimin Wang & Ronggui Li

  2. The Second Hospital of Jilin University, Changchun, People’s Republic of China

    Shanshan Qu & Zonggui Wang

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  1. Haiying Zhang
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  2. Shanshan Qu
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  3. Shuang Li
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  4. Yang Wang
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  5. Yulin Li
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  6. Yimin Wang
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  7. Zonggui Wang
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  8. Ronggui Li
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Correspondence to Zonggui Wang or Ronggui Li.

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Zhang, H., Qu, S., Li, S. et al. Silencing SATB1 inhibits proliferation of human osteosarcoma U2OS cells. Mol Cell Biochem 378, 39–45 (2013). https://doi.org/10.1007/s11010-013-1591-0

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  • DOI: https://doi.org/10.1007/s11010-013-1591-0

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