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Correlation of SATB1 overexpression with the progression of human rectal cancer

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Background and aims

To date, the association between special AT-rich sequence-binding protein 1 (SATB1) and colorectal cancer (CRC) has not been reported. This study was aimed at investigating the expression and potential role of SATB1 in human rectal cancers.

Methods

Ninety-three paired samples of rectal cancer and distant normal rectal tissue were analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), and the correlations between SATB1 expression and clinicopathological parameters were evaluated. The expression profiles of SATB1 were also investigated in a panel of five human colon carcinoma cell lines.

Results

The general level of SATB1 mRNA in rectal cancer tissues was statistically significantly higher than that in normal mucosa (P = 0.043). The rate of positive SATB1 protein expression in rectal cancers (44.1%) was significantly higher than that in normal tissues (25.8%) by IHC analysis (P = 0.009). Overexpression of SATB1 mRNA was more predominant in patients with earlier onset of rectal cancer (P = 0.033). SATB1 expression correlated with invasive depth and tumor node metastasis (TNM) stage at both protein and mRNA levels (P < 0.05). Furthermore, SATB1 expression in the poorly metastatic KM12C cells was significantly lower than the highly metastatic KM12SM and KM12L4A cells and higher than the HCT116 and SW480 cells (P = 0.001). These results were further confirmed by Western blotting.

Conclusion

Our results indicate that SATB1 may play an important role in the progression of human rectal cancer, which represents a possible new mechanism underlying CRC.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 30830100) and Ph.D. Programs Foundation of the Ministry of Education of China (No. 20080610058). We thank Yong Liu and Yang Li for their technical assistance.

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, 610041, China

    Wen-Jian Meng, Hui Yan, Wei Zhang, Xiang-Heng Kong & Zong-Guang Zhou

  2. Institute of Digestive Surgery and Organ Microcirculation, West China Hospital, Sichuan University, 1 Keyuan Road 4, Chengdu, 610041, China

    Wen-Jian Meng, Hui Yan, Bin Zhou, Wei Zhang, Xiang-Heng Kong, Rong Wang, Lan Zhan, Yuan Li, Zong-Guang Zhou & Xiao-Feng Sun

  3. Department of Pediatric Surgery, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, 610041, China

    Yuan Li

  4. National Key Laboratory of Biotherapy of West China Hospital, Sichuan University, 1 Keyuan Road 4, Chengdu, 610041, China

    Yuan Li & Zong-Guang Zhou

  5. Department of Oncology, Institute of Clinical and Experimental Medicine, Linköping University, S-581 85, Linköping, Sweden

    Xiao-Feng Sun

Authors
  1. Wen-Jian Meng
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  2. Hui Yan
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  3. Bin Zhou
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  4. Wei Zhang
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  6. Rong Wang
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  7. Lan Zhan
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  8. Yuan Li
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  9. Zong-Guang Zhou
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  10. Xiao-Feng Sun
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Corresponding author

Correspondence to Zong-Guang Zhou.

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Fig. S1

The relative expressions of SATB1 mRNA in five colon cancer cell lines detected by qRT-PCR. The amplification curves and corresponding Ct values of SATB1 and β-actin gene (reference gene) are shown. The experiment was repeated three times (JPEG 61 kb)

High resolution image (TIFF 539 kb)

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Meng, WJ., Yan, H., Zhou, B. et al. Correlation of SATB1 overexpression with the progression of human rectal cancer. Int J Colorectal Dis 27, 143–150 (2012). https://doi.org/10.1007/s00384-011-1302-9

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  • DOI: https://doi.org/10.1007/s00384-011-1302-9

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