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Examination of typical bacterial strains in septic arthritis by isoperibol calorimeter

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Abstract

The accurate diagnosis of different types of arthritis is a great clinical challenge. It has a special importance to reach the right diagnosis in case of septic joint infections. The clarification of aetiology is crucial to minimize the complications and mortality of these cases. Therefore, novel methods like synovial sample analyses by differential scanning calorimetry (DSC) could have the potential to provide further information in the diagnostic process (Dandé et al. in J Therm Anal Calorim. doi:https://doi.org/10.1007/s10973-017-6179-6, 2017). A retrospective analysis of patients treated with septic joint infections over the last 5 years at the Department of Traumatology and Hand Surgery has been conducted. Human synovial samples were inoculated with six different, frequently occurring human pathogenic bacterial strains. The proliferation of inoculated synovial fluid samples was monitored by SETARAM Micro DSC-II calorimeter in isotherm mode (isoperibol calorimeter). Based on our results, 47.45% of the cases involved small joint (SG) infections and 52.55% of them occurred in large joints (LG). Synovial aspirates collected from infected joints indicated the presence of the following bacteria and incidences: Staphylococcus aureus 43 (22.16%), Staphylococcus epidermidis 3 (1.54%), Methicillin-resistant Staphylococcus aureus, MRSA 4 (2.06%), Streptococcus pyogenes 9 (4.63%), Escherichia coli 1 (0.51%) and Pseudomonas aeruginosa 4 (2.06%). The isoperibolic curves of human synovial samples inoculated with different bacterial strains clearly demonstrated specific/representative results in case of each bacterium. The control of human synovial fluid samples by isotherm calorimetry could be a useful tool in the differential diagnosis of human septic arthritis.

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Acknowledgements

This work was supported by Grant OTKA CO-272 (for D. Lőrinczy). The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.

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Authors and Affiliations

  1. Department of Traumatology and Hand Surgery, Faculty of Medicine, University of Pécs, Szigeti str. 12, Pecs, 7624, Hungary

    Á. Dandé, L. G. Nőt, G. Bűcs & N. Wiegand

  2. Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti str. 12, Pecs, 7624, Hungary

    B. Kocsis

  3. Department of Biophysics, Institute of Biophysics School of Medicine, Faculty of Medicine, University of Pécs, Szigeti str. 12, Pecs, 7624, Hungary

    D. Lőrinczy

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  1. Á. Dandé
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  2. L. G. Nőt
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  4. N. Wiegand
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Correspondence to D. Lőrinczy.

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Dandé, Á., Nőt, L.G., Bűcs, G. et al. Examination of typical bacterial strains in septic arthritis by isoperibol calorimeter. J Therm Anal Calorim 131, 2041–2048 (2018). https://doi.org/10.1007/s10973-017-6859-2

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  • DOI: https://doi.org/10.1007/s10973-017-6859-2

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