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DSC study of the association of ethanol with human serum albumin

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Abstract

Human serum albumin unfolding in ethanol/water mixtures was studied by use of differential scanning calorimetry. Ethanol-induced changes in DSC curves of defatted and non-defatted albumin were markedly different. In the presence of ethanol, bimodal denaturation transition for fatty acid free albumin was observed while that for albumin containing endogenous fatty acids was single and more sharpen than in aqueous solution. Ethanol was found to decrease the thermal stability of albumin due to the binding to the unfolded state to a higher degree than to the native state, thus favouring unfolding. The binding with different affinities has been suggested depending on ethanol concentration range.

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Authors and Affiliations

  1. A. Chełkowski’ Institute of Physics, Department of Medical Physics, University of Silesia, Katowice, Poland, 40-007, ul. Uniwerytecka 4

    Anna Michnik

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  1. Anna Michnik
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Correspondence to Anna Michnik.

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Michnik, A. DSC study of the association of ethanol with human serum albumin. J Therm Anal Calorim 87, 91–96 (2007). https://doi.org/10.1007/s10973-006-7825-6

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  • DOI: https://doi.org/10.1007/s10973-006-7825-6

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