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Evidences for the use of 14C content in the root exudates as a novel application of radiocarbon labelling for screening iron deficiency tolerance of soybean (Glycine max (L.) Merr.) genotypes

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Abstract

Four genotypes of soybean viz. NRC-45, IC-18734, J-231 and G-2132, which differ in their inherent ability to tolerate iron limiting stress, were selected as representative genotypes for iron efficient and responsive (FeER), iron inefficient and responsive (FeIR), iron efficient and nonresponsive (FeENR) and iron inefficient and nonresponsive (FeINR) categories, respectively. The relative performance of these genotypes to iron limiting stress was evaluated in hydroponics experiment with low iron (−Fe) and sufficient iron (+Fe). We hypothesised that the genotype exhibiting higher iron deficiency tolerance would exhibit greater root exudation capacity, which in turn can be screened through radiocarbon labelling technique, by counting the 14C content in the total root exudates (14CTRE). The genotype FeER (NRC-45) exuded out significantly higher amount of 14C in the total root exudates with the lower and upper bound values within 95% confidence interval (CI) were 151.9 and 163.6 Bq g−1 fw root−1 (FeER: NRC-45), followed by 138.5 and 150.1 Bq g−1 fw root−1 (FeENR: J-231), 86.5 and 98.1 Bq g−1 fw root−1 (FeIR: IC-18734), and 63.3 and 74.9 Bq g−1 fw root−1 (FeINR: G-2132) under Fe deficient (−Fe) condition.

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Acknowledgements

The authors are thankful to the International Plant Nutrition Institute (IPNI), USA; University Grant Commission (UGC) of India, Post Graduate School ICAR-Indian Agricultural Research Institute, New Delhi and National Phytotron Facility (NPF), New Delhi for providing necessary support.

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Authors and Affiliations

  1. Soil Science and Agricultural Chemistry, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Kiran Karthik Raj, R. N. Pandey & M. C. Meena

  2. Natural Resource Management, ICAR-Central Island Agricultural Research Institute, Port Blair, 744105, India

    Kiran Karthik Raj

  3. Nuclear Research Laboratory, Centre for Environment Science and Climate Resilient Agriculture (CESCRA), ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Bhupinder Singh

  4. Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    A. Talukdar

  5. Radio Tracer Laboratory, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Kapil Atmaram Chobhe

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Kiran Karthik Raj, Pandey, R.N., Singh, B. et al. Evidences for the use of 14C content in the root exudates as a novel application of radiocarbon labelling for screening iron deficiency tolerance of soybean (Glycine max (L.) Merr.) genotypes. J Radioanal Nucl Chem 326, 487–496 (2020). https://doi.org/10.1007/s10967-020-07284-5

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