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14C labelling as a reliable technique to screen soybean genotypes (Glycine max (L.) Merr.) for iron deficiency tolerance

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Abstract

Fifty diverse soybean genotypes were screened for their ability to tolerate iron deficiency stress in a hydroponics experiment with low iron (−Fe) and sufficient iron (+Fe). We hypothesised that the genotypes with higher root exudation potential would exhibit higher chlorophyll content, dry matter production and Fe acquisition. The relative root exudation capacity of the genotypes was estimated with the help of 14C. As compared to iron inefficient and non responsive (FeINR) category under sufficient availability of iron (+Fe), the average 14C content in the total root exudates (14CTRE) was 39.4% higher in iron efficient and responsive (FeER) category. Further, higher exudation was observed under iron limiting (−Fe) stress condition and reported maximum in FeER (110.0% increase over FeINR under +Fe condition). The strength of positive correlation between 14C released with other parameters related to iron deficiency chlorosis suggested that 14C could be effectively used as a tracer for providing reliable estimate for better screening of iron efficient and responsive categories of soybean genotypes.

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Authors and Affiliations

  1. Soil Science and Agricultural Chemistry, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Kiran Karthik Raj & R. N. Pandey

  2. Nuclear Research Laboratory, Centre for Environment Science and Climate Resilient Agriculture (CESCRA), ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Bhupinder Singh

  3. Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    A. Talukdar

  4. Natural Resource Management, ICAR-Central Island Agricultural Research Institute, Port Blair, 744105, India

    Kiran Karthik Raj

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  1. Kiran Karthik Raj
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  2. R. N. Pandey
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  3. Bhupinder Singh
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  4. A. Talukdar
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Correspondence to Kiran Karthik Raj.

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Appendix

Appendix

See Tables 2 and 3.

Table 2 Average response of soybean genotypes (20 DAS) grown in iron sufficient (+Fe) and deficient (−Fe) nutrient cultures
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Table 3 Relative dry matter production and 14C exudation by genotypes of soybean
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Raj, K.K., Pandey, R.N., Singh, B. et al. 14C labelling as a reliable technique to screen soybean genotypes (Glycine max (L.) Merr.) for iron deficiency tolerance. J Radioanal Nucl Chem 322, 655–662 (2019). https://doi.org/10.1007/s10967-019-06708-1

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  • DOI: https://doi.org/10.1007/s10967-019-06708-1

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