Abstract
The binding of riboflavin (RBF) to norfloxacin (NF) was investigated by spectroscopic techniques. The results revealed that RBF caused the fluorescence quenching of NF by complex formation. The binding parameters and corresponding thermodynamic parameters ΔH, ΔG and ΔS at different temperatures were calculated. The negative enthalpy (ΔH) and positive entropy (ΔS) values indicated that both hydrogen bond and hydrophobic forces played major roles in the binding of RBF to NF. The distance r between donor (NF) and acceptor (RBF) was obtained according to the Forster’s theory of non-radiative energy transfer. The method of quenching was successfully applied for the determination of riboflavin from pharmaceutical samples.
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More, V.R., Mote, U.S., Patil, S.R. et al. Fluorescence Quenching Studies of the Interaction Between Riboflavin and Norfloxacin and Analytical Application in the Determination of Vitamin B2 . J Solution Chem 39, 97–106 (2010). https://doi.org/10.1007/s10953-009-9490-4
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DOI: https://doi.org/10.1007/s10953-009-9490-4