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Comparative Appraisal of Leaf Proteomic and Mass Spectrometry Analyses During Fusarium Wilt Infection in Resistance and Susceptible Genotypes of Castor (Ricinus communis L.)

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Abstract

The resistant and susceptible genotypes of castor were utilized for leaf proteomic study during Fusarium wilt infection. The histopathological study was observed under SEM and it confirmed that the infection of Fusarium oxysporum f. sp. ricini was higher in the root of susceptible JI-35, while incompatible interaction is observed in resistant SKI-215 genotype. The acidic and neutral proteins were maximally up-expressed with 2 to 171 kDa in treated resistant and 2 to 150 kDa in treated susceptible interactions. In resistant genotype, the leaf proteins were recognized with 3.0- and 5.8-fold higher at infection stage and post-infection stage, respectively, as compared to susceptible genotype. The highly up expressions of leaf acidic (4.76 pI) and basic (8.77 pI) proteins were found with 224.94- and 61.68-fold change, respectively during the post-infection stage in treated resistance compared to its control. The protein spots at 4.76 pI and 8.77 pI were characterized with nanoLC–MS Triple TOF and were recognized as signalling molecules small GTP binding protein (23 kDa) and actin (8 kDa), respectively, on the basis of mass spectrometry and peptide sequences. However, basic and neutral proteins were up regulated as 30.11- and 20.30-fold changes in treated susceptible compared to its control. These proteins were identified as HSP90 (10 kDa) and LEA (27 kDa) proteins. The 148 kDa protein is recognized as histidine kinase in incompatible resistant interaction compared to compatible susceptible (serine threonine protein kinase, 65 kDa) as common acidic protein at 3.80 pI during infection stage. Some acidic proteins were maximally up-regulated in the leaf of resistant castor genotype and played a significant role in defense response.

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The data that support this study will be shared upon reasonable request to the corresponding author.

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Authors and Affiliations

  1. Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, 362 001, India

    Harshita J. Kachhadiya, H. P. Gajera, D. R. Mehta, Darshna G. Hirpara, Rushita V. Bhadani & R. A. Dave

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  1. Harshita J. Kachhadiya
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  2. H. P. Gajera
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  3. D. R. Mehta
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  4. Darshna G. Hirpara
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  5. Rushita V. Bhadani
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  6. R. A. Dave
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Contributions

HJK carried out the experiment, microbial work, biochemical analysis, and worked out the results; HPG was responsible for interpretation of data, writing of the manuscript and designing the experiments. DRM was liable for the idea and coordination of the experiment. DGH and RVB responsible for helping in microbial and biochemical analyses; and analyzed the data. RAD carried out nanoLC–MS analysis. All authors contributed critically to the drafts and gave final approval for publication.

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Correspondence to H. P. Gajera.

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Kachhadiya, H.J., Gajera, H.P., Mehta, D.R. et al. Comparative Appraisal of Leaf Proteomic and Mass Spectrometry Analyses During Fusarium Wilt Infection in Resistance and Susceptible Genotypes of Castor (Ricinus communis L.). Protein J 41, 638–658 (2022). https://doi.org/10.1007/s10930-022-10083-4

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