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Proteomics analysis of sensitive and tolerant barley genotypes under drought stress

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Abstract

Drought is a severe environmental constraint to plant productivity and an important factor limiting barley yield. To investigate the initial response of barley to drought stress, changes in protein profile were analyzed using a proteomics technique. Three-day-old barley seedlings of sensitive genotype 004186 and tolerant genotype 004223 were given two treatments, one with 20 % polyethylene glycol and the second with drought induced by withholding water. After 3 days of treatments, proteins were extracted from shoots and separated by 2-dimensional polyacrylamide gel electrophoresis. Metabolism related proteins were decreased in the sensitive genotype under drought; however, they were increased in the tolerant genotype. Photosynthetic related proteins were decreased and increased among the three sensitive and three tolerant genotypes, respectively. In addition, amino acid synthesis and degradation related proteins were increased and decreased among the three tolerant genotypes. These results suggest that chloroplastic metabolism and energy related proteins might play a significant role in the adaptation process of barley seedlings under drought stress.

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Abbreviations

2-DE:

Two-dimensional polyacrylamide gel electrophoresis

CBB:

Coomassie brilliant blue

MS:

Mass spectrometry

LC:

Liquid chromatography

pI:

Isoelectric point

IEF:

Isoelectric focusing

PEG:

Polyethylene glycol

ROS:

Reactive oxygen species

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Acknowledgments

The authors thank Dr. Yohei Nanjo, Dr. Keito Nishizawa and Mr. Mehdi Ghaffari at National Institute of Crop Science for their valuable comments and discussion. We also thank Dr. Fayyaz-ul-Hassan Sahi and Dr. Rehmatullah Qureshi for their valuable suggestions. We thank Higher Education Commission of Pakistan for awarding International Research Support Initiative Scholarship grant and National Institute of Crop Science, Japan for providing the research facilities.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Authors and Affiliations

  1. National Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan

    Rehana Kausar & Setsuko Komatsu

  2. Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, 46300, Pakistan

    Rehana Kausar & Muhammad Arshad

  3. Plant Biotechnology Program, National Agriculture Research Centre, Islamabad, 45500, Pakistan

    Armghan Shahzad

Authors
  1. Rehana Kausar
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  2. Muhammad Arshad
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  3. Armghan Shahzad
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  4. Setsuko Komatsu
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Corresponding author

Correspondence to Setsuko Komatsu.

Electronic supplementary material

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726_2012_1338_MOESM1_ESM.pptx

Supplementary Fig. 1 Photograph of the barley genotypes grown under PEG treatment. Six-day-old barley seedlings were treated with 10 % and 20 % PEG until 26-day-old plantlets. Physiological data taken were used for cluster analysis. The experiment was performed in three replicates. (PPTX 380 kb)

726_2012_1338_MOESM2_ESM.pptx

Supplementary Fig. 2 Effects of drought stress on changed proteins in 3 sensitive and 3 tolerant barley genotypes. Differentially expressed proteins of 3 sensitive 004186, Jau-83 and Sanober-96 (A) and 3 tolerant 004223, 004360 and Frontier-87 (B) barley genotypes without (white columns) and with drought (black columns) were analyzed. Values are the mean ± SE from 3 independent experiments and asterisks * and ** indicate significant differences at p < 0.05 and 0.01, respectively. (PPTX 42 kb)

Supplementary material 3 (DOCX 14 kb)

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Kausar, R., Arshad, M., Shahzad, A. et al. Proteomics analysis of sensitive and tolerant barley genotypes under drought stress. Amino Acids 44, 345–359 (2013). https://doi.org/10.1007/s00726-012-1338-3

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  • DOI: https://doi.org/10.1007/s00726-012-1338-3

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