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Molecular and Enzymatic Characterization of 9-Cis-epoxycarotenoid Dioxygenases from Mulberry

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Abstract

Abscisic acid (ABA) is involved in many physiological regulatory processes in plants, such as leaf shedding, stomatal closure, inhibition of cell elongation, as well as responses to multi-abiotic stress, and 9-cis epoxy carotenoid dioxygenase (NCED) is related to the indirect synthesis of ABA. However, NCED genes involved in multi-abiotic stress and ABA synthesis pathway in mulberry (Morus alba L.) are still unknown. Here, two NCED genes cloned from mulberry (MaNCED) and their function were preliminarily identified. Interestingly, MaNCED2 responded strongly to drought stress while MaNCED1 responded strongly to pathogen stress. Then, two MaNCED proteins were successfully obtained by prokaryotic expression, and the degradation products of MaNCED1 and MaNCED2 were analyzed using UPLC-MS. The results show that recombinant MaNCED1 and MaNCED2 both cleave 9-cis-violaxanthin to form C15 xanthoxin, involved in the formation of the precursor of ABA.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Guangxi Science and Technology Major Special Project, Grant Number (Guike AA19182012-2).

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Authors and Affiliations

  1. Sericulture Technology Extension Station of Guangxi Zhuang Autonomous Region, NanNing, 530000, China

    Dan Liu, Changyu Qiu, Yanrong Zeng & Qiang Lin

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  1. Dan Liu
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  2. Changyu Qiu
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  3. Yanrong Zeng
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  4. Qiang Lin
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Contributions

QL conceived and designed the study. DL performed most of the experiments and wrote the manuscript. CQ and YZ performed the bioinformatics and analyzed the data of this work.

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Correspondence to Dan Liu or Qiang Lin.

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Liu, D., Qiu, C., Zeng, Y. et al. Molecular and Enzymatic Characterization of 9-Cis-epoxycarotenoid Dioxygenases from Mulberry. Protein J 41, 504–514 (2022). https://doi.org/10.1007/s10930-022-10072-7

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