Abstract
A new turn on fluorescence probe based on 3′,6′-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-(p-tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one (BFFPH) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of BFFPH for monitoring of pH change in alkaline medium was studied. BFFPH exhibited a high sensitivity to alkaline pH by two pKa values at 8.82 and 10.66 in UV/vis spectroscopy titration. The pH monitoring was studied in broad range of pH values (2.5–12.2) at two pKa values at 8.72 and 10.73 by recording the effect of pH on the fluorescence intensity of BFFPH. The acid-base reversibility character of the probe was investigated as well as the effect of the pH change on the fluorescence quantum yield. The application of the prepared BFFPH probe for detection of living Escherichia coli (E. coli) bacteria using confocal fluorescence microscope was investigated.
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Acknowledgements
We gratefully thanks National Research Centre, Giza, Egypt through project 11070103 (colorimetric chemosensors and fluorescence probes: a promising strategy for environmental monitoring of ionic species and toxic gases) for the financial support. We could not forget to thank the centre of excellence for advances sciences, National Research Centre and the central laboratory of textile research division for allowing us to use all their apparatus. We also thank the Sensors-Probes and Devices Lab, Chemistry Department, Faculty of Science, Al-Azhar University to produce this work. Also we thank Prof. Dr. Omaima M. Kandil director to Embryo and Genetic Resource Conservation Bank in National Research Centre, financially supported by STDF (C.B. grant ID: 2339) for her work in preparation of samples for image application using confocal microscope zeiss LSM 710 photos.
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Mohamed, M.B.I., Aysha, T.S., Elmorsi, T.M. et al. Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria. J Fluoresc 30, 601–612 (2020). https://doi.org/10.1007/s10895-020-02522-1
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DOI: https://doi.org/10.1007/s10895-020-02522-1