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Pyrene Based Fluorescent Turn-on Chemosensor for Sequential Detection of Fe3+ and Fe2+ Ions and its Application in Live Cell Imaging

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Abstract

A simple pyrene-based “turn-on” chemosensor bearing isonizide namely 3-(pyren-1-yl methylene) pentane-2,4-diyldene di(isonicotinohydrazide) (PMPD) was designed and synthesized for detecting Fe3+and Fe2+ ions in HEPES buffer solution at pH 7.4 (DMSO: H2O (1:9 v/v)) medium. The probe shows conducive selectivity for Fe2+ and Fe3+ ions over the other competitive metal cations. The detection limit was found to be 1.67 μM for Fe3+ and 2.02 μM for Fe2+. The recognition mechanism of PMPD towards Fe3+ and Fe2+ has been examined in detail by absorption, emission, and ESI-MS studies. Moreover, “turn-on” fluorescence behavior of the probe was used to track iron ions in living cells.

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Acknowledgements

The authors express their sincere thanks to Council of Scientific and Industrial Research (CSIR), New Delhi, India [Grant No. 01(2907)/17/EMR-II] for financial support.

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Authors and Affiliations

  1. Department of Chemistry, Periyar University, Salem, 636 011, India

    Rajasekaran Dhivya, Asaithambi Gomathi & Periasamy Viswanathamurthi

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  1. Rajasekaran Dhivya
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  2. Asaithambi Gomathi
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  3. Periasamy Viswanathamurthi
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Correspondence to Periasamy Viswanathamurthi.

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Dhivya, R., Gomathi, A. & Viswanathamurthi, P. Pyrene Based Fluorescent Turn-on Chemosensor for Sequential Detection of Fe3+ and Fe2+ Ions and its Application in Live Cell Imaging. J Fluoresc 29, 797–802 (2019). https://doi.org/10.1007/s10895-019-02392-2

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