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Physicochemical Characterization and In Vitro Cytotoxic Effect of 3-Hydroxyflavone in a Silver Nanoparticles Complex

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Abstract

The aim of this work was to characterize the physico-chemical properties of 3-hydroxyflavone (3-HF) in a silver nanoparticles complex (SNPs) using UV–vis and Fluorescence spectroscopy, Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM) analysis. One also evaluated its effect on the cell viability and morphology of L929 mouse fibroblast cells in vitro. The contribution of the carrier protein, Bovine Serum Albumin (BSA) to 3-HF properties has also been investigated. 3-HF in BSA/SNPs systems presented no cytotoxic effect in L929 mouse fibroblast cells at any of the tested concentrations. The results are discussed with relevance to the oxidative stress process.

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Acknowledgments

This work was supported by a grant of the Romanian National Authority for Scientific Research, CNCS – UEFISCDI, project number PN-II-RU-TE-2012-3-0055.

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Authors and Affiliations

  1. Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, Splaiul Independentei 202, 060021, Bucharest, Romania

    Mariana Voicescu, Mihai Anastasescu & Daniel G. Angelescu

  2. National Institute of R&D for Biological Sciences, Splaiul Independentei 296, 060031, Bucharest, Romania

    Oana Craciunescu & Lucia Moldovan

  3. Institute of Atomic Physics, National Institute of Materials Physics, Magurele, 077125, Romania

    Valentin S. Teodorescu

Authors
  1. Mariana Voicescu
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  2. Oana Craciunescu
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  3. Lucia Moldovan
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  4. Mihai Anastasescu
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  5. Daniel G. Angelescu
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  6. Valentin S. Teodorescu
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Correspondence to Mariana Voicescu.

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Voicescu, M., Craciunescu, O., Moldovan, L. et al. Physicochemical Characterization and In Vitro Cytotoxic Effect of 3-Hydroxyflavone in a Silver Nanoparticles Complex. J Fluoresc 25, 1215–1223 (2015). https://doi.org/10.1007/s10895-015-1608-z

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  • DOI: https://doi.org/10.1007/s10895-015-1608-z

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