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Spectroscopic and coarse-grained simulation studies of the BSA and HSA protein adsorption on silver nanoparticles

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Abstract

The photophysical properties of the bovine serum albumin (BSA) and human serum albumin (HSA) adsorbed on (non) functionalized Ag(0) nanoparticles have been studied by spectroscopic techniques. The surface plasmon resonance kinetic of the BSA/HSA-Ag(0) nanoparticle complexes has been assessed by UV–Vis absorption spectroscopy. Transmission electron microscopy analysis showed that the average size of the particles is ~9 nm and the core–shell structure of the protein-Ag(0) nanoparticle complexes has been supported by UV–Vis spectra. The structure, stability, dynamics, and conformation of the proteins have been investigated by steady-state, time-resolved fluorescence, and circular dichroism spectroscopy. Insights of the HSA conformation at the nanoparticle surface were obtained by the Monte Carlo simulations carried out using an appropriate coarse-grained model. The HSA conformation upon adsorption on the nanoparticle surface is distorted so that the Trp fluorescence is quenched and the α-helix content diminished. The adsorbed protein exhibited an extended conformation with Trp residue depleted from the nanoparticle surface and rather located toward the protein boundary. Experimental and simulated experiments were in good agreements and the results are discussed in terms of functional properties of the serum albumins in protein–Ag(0) nanoparticle complex.

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Acknowledgments

This study has been performed in the frame of the Romanian Academy programme and II. 8 theme of the INFRANANOCHEM Project. DA acknowledges the use of the HPC infrastructure developed under NASR Grant, Capacities Project CpI 84/2007. The authors are indebted to Dr. Valentin S. Teodorescu for Transmission Electron Microscopy.

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Authors and Affiliations

  1. Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, Splaiul Independentei 202, 060021, Bucharest, Romania

    Mariana Voicescu & Daniel G. Angelescu

  2. Department of Physical Chemistry, University of Bucharest, Bd Regina Elisabeta 4-12, 030018, Bucharest, Romania

    Sorana Ionescu

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  1. Mariana Voicescu
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  2. Sorana Ionescu
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  3. Daniel G. Angelescu
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Correspondence to Mariana Voicescu.

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Voicescu, M., Ionescu, S. & Angelescu, D.G. Spectroscopic and coarse-grained simulation studies of the BSA and HSA protein adsorption on silver nanoparticles. J Nanopart Res 14, 1174 (2012). https://doi.org/10.1007/s11051-012-1174-0

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