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A Pyrene-based Highly Selective Turn-on Fluorescent Chemosensor for Iron(III) Ions and its Application in Living Cell Imaging

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Abstract

A new pyrene-based chemosensor (1) exhibits excellent selectivity for Fe3+ ions over a wide range of tested metal ions Ag+, Ca2+, Cd2+, Co2+, Cu2+, Fe2+, Hg2+, K+, Mg2+, Mn2+, Ni2+, Pb2+, and Zn2+. The binding of Fe3+ to chemosensor 1 produces an emission band at 507 nm due to the formation of a Py-Py* excimer that is induced by Fe3+-binding. The binding ratio of 1-Fe3+ was determined to be 1:1 from a Job plot. The association constant of 1-Fe3+ complexes was found to be 1.27 × 104 M−1 from a Benesi-Hildebrand plot. In addition, fluorescence microscopy experiments show that 1 can be used as a fluorescent probe for detecting Fe3+ in living cells.

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Acknowledgments

We gratefully acknowledge the financial supports of the National Science Council (ROC) and National Chiao Tung University.

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Authors and Affiliations

  1. Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300, Republic of China

    Peter Kun Chung, Shi-Rong Liu, Hsuan-Fu Wang & Shu-Pao Wu

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  1. Peter Kun Chung
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  2. Shi-Rong Liu
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  3. Hsuan-Fu Wang
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  4. Shu-Pao Wu
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Correspondence to Shu-Pao Wu.

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Chung, P.K., Liu, SR., Wang, HF. et al. A Pyrene-based Highly Selective Turn-on Fluorescent Chemosensor for Iron(III) Ions and its Application in Living Cell Imaging. J Fluoresc 23, 1139–1145 (2013). https://doi.org/10.1007/s10895-013-1242-6

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  • DOI: https://doi.org/10.1007/s10895-013-1242-6

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