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Terpyridine derivatives as “turn-on” fluorescence chemosensors for the selective and sensitive detection of Zn2+ ions in solution and in live cells

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Abstract

A terpyridine based compound L1 was designed and synthesized as an “off-on” chemosensor for the detection of Zn2+. Chemosensor L1 showed excellent selectivity and sensitivity toward Zn2+ by exhibiting a large fluorescence enhancement (~51-fold) at 370 nm whereas other competitive metal ions did not show any noticeable change in the emission spectra of chemosensor L1. The chemosensor (L1)was shown to detect Zn2+ ions down to 9.76 μM at pH 7.4. However, chemosensor L1 binds Zn2+ in a 1: 2 ratio (receptor: metal) with an association constant of 1.85 x 104 (R2 = 0.993) and this 1: 2 stoichiometric fashion is established on the basis of a Job plot and mass spectroscopy. DFT/TD-DFT calculations were carried out to understand the binding nature, coordination features and electronic properties of L1 and the L1-2Zn2+ complex. In addition, this “turn-on” fluorescence probe was effectively used to image intracellular Zn2+ ions in cultured MDA-MB-468 cells.

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Acknowledgements

TM and AH thankfully acknowledge the UGC, New Delhi for senior research fellowships. SP and SKM thank Haldia Government College for laboratory facility. AD is grateful to UGC, India for the DSK Postdoctoral fellowship.

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Authors and Affiliations

  1. Department of Chemistry, Jadavpur University, Kolkata, 700032, India

    Tripti Mandal, Anowar Hossain, Anamika Dhara & Subrata Mukhopadhyay

  2. Department of Life Science & Bio-technology, Jadavpur University, Kolkata, 700032, India

    Abdulla Al Masum

  3. Department ofChemistry, The Bhawanipur Education Society College, Kolkata, 700 020, India

    Saugata Konar

  4. Department ofChemistry, Haldia Government College, Debhog, Purba Medinipur, West Bengal, 721657, India

    Saikat Kumar Manna & Sudipta Pathak

  5. Department ofPhysics, Jadavpur University, Kolkata, 700032, India

    Saikat Kumar Seth

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  1. Tripti Mandal
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Mandal, T., Hossain, A., Dhara, A. et al. Terpyridine derivatives as “turn-on” fluorescence chemosensors for the selective and sensitive detection of Zn2+ ions in solution and in live cells. Photochem Photobiol Sci 17, 1068–1074 (2018). https://doi.org/10.1039/c8pp00186c

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