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Nano Biomedical Potential of Biopolymer Chitosan-Capped Silver Nanoparticles with Special Reference to Antibacterial, Antibiofilm, Anticoagulant and Wound Dressing Material

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Abstract

Silver nanoparticles have been widely used to treat bacterial infections on wounds. Chitosan is a natural polymer having good biocompatibility and biodegradability. Chitosan have antibacterial effects and are largely used in healthcare practices to cure wounds. In this scenario, an attempt was made to biologically synthesize AgNPs using the chitosan extracted from shrimp shell waste. The synthesized Ch-AgNPs exhibited strong UV absorption spectra at 404 nm. XRD spectrum confirmed the presence of nanocrystals with an average crystallite size of 26 nm. HR-TEM images have shown spherical shaped particles with the size ranging between 10 and 50 nm. Ch-AgNPs exhibited antibacterial activity in a dose-dependent manner and greater activity was observed against P. aeruginosa. The zone of inhibition against P. aeruginosa was 10, 15 and 20 mm at 25, 50 and 100 µg mL−1, respectively. The zone of inhibition against MRSA was 8, 15 and 18 mm at 25, 50 and 100 µg mL−1 respectively. Ch-AgNPs effectively inhibited the biofilm of MRSA and P. aeruginosa at 100 µg mL−1. In addition, Ch-AgNPs coated band-aid cloth showed greater antibacterial activity compared to uncoated band-aid cloth. Furthermore, Ch-AgNPs exhibited anticoagulant activity by inhibiting the coagulation of human blood for 24 h.

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References

  1. R. S. Kirsner, H. Orstead, and J. B. Wright (2001). Wounds.13, 5–12.

    Google Scholar 

  2. S. L. Percival, S. M. McCarty, and B. Lipsky (2015). Adv. Wound Care.4, 373–381.

    Article  Google Scholar 

  3. S. S. Grant and D. T. Hung (2013). Virulence.4, 273–283.

    Article  PubMed  PubMed Central  Google Scholar 

  4. C. E. Black and J. W. Costerton (2010). Surg. Clin. N. Am.90, 1147–1160.

    Article  PubMed  Google Scholar 

  5. S. Finnegan and S. L. Percival (2015). Adv. Wound Care4, 398–406.

    Article  Google Scholar 

  6. R. Edwards and K. G. Harding (2004). Curr. Opin. Infect. Dis.17, (2), 91–96.

    Article  PubMed  Google Scholar 

  7. L. Scrivano, D. Iacopetta, M. S. Sinicropi, C. Saturnino, P. Longo, O. I. Parisi, and F. Puoci (2017). Drug Deliv.24, 482–490.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. R. Jayakumar, M. Prabaharan, and R. A. A. Muzzarelli (2011). Adv. Polym. Sci.243, 23–54.

    Article  CAS  Google Scholar 

  9. M. Dash, F. Chiellini, R. M. Ottenbrite, and E. Chiellini (2011). Prog. Polym. Sci.36, 981–1014.

    Article  CAS  Google Scholar 

  10. M.I.Z. Lionzo, A.C. Dressler, O. Mertins, A.R. Pohlmann and N.P. da Silveira, in Nanocosmetics and Nanomedicines (2011), pp. 163–174 (Chapter 8).

    Chapter  Google Scholar 

  11. M. Kong, X. G. Chen, K. Xing, and H. J. Park (2010). Int. J. Food Microbiol.144, 51–63.

    Article  CAS  PubMed  Google Scholar 

  12. R. Jayakumar, M. Prabaharan, P. T. Sudheesh Kumar, S. V. Nair, and H. Tamura (2011). Biotechnol. Adv.29, 322–337.

    Article  CAS  PubMed  Google Scholar 

  13. X. Chen and H. J. Schluesener (2008). Toxicol. Lett.176, 1–12.

    Article  CAS  PubMed  Google Scholar 

  14. S. Vijayakumar, B. Malaikozhundan, N. Gobi, B. Vaseeharan, and C. Murthy (2016). Limnologica.61, 44–51.

    Article  CAS  Google Scholar 

  15. D. Bagal-Kestwal, R. M. Kestwal, W. T. Hsieh, and B. H. Chiang (2014). J. Pharm. Biomed. Anal.88, 571–578.

    Article  CAS  PubMed  Google Scholar 

  16. B. Ankamwar, M. Chaudhary, and M. Sastry (2005). Metal Organic Nano Metal Chem.35, 19–26.

    Article  CAS  Google Scholar 

  17. S. Shankar, A. Absar, and S. Murali (2003). Biotech. Prog.19, 1627–1631.

    Article  CAS  Google Scholar 

  18. M. Ali, N. Thajuddin, K. Jeganathan, and M. Gunasekaran (2011). Colloids Surf. B.85, 360–365.

    Article  CAS  Google Scholar 

  19. S. P. Chandran, M. Chaudhary, R. Pasricha, A. M. Ahmad, and M. Sastry (2006). Biotech. Prog.22, 577–583.

    Article  CAS  Google Scholar 

  20. V. Deepak, P. S. Umamaheshwaran, K. Guhan, R. A. Nanthini, B. Krithiga, N. M. H. Jaithoon, and S. Gurunathan (2011). Colloids Surf. B.86, 353–358.

    Article  CAS  Google Scholar 

  21. P. Jena, S. Mohanty, R. Mallick, B. Jacob, and A. Sonawane (2012). Int. J. Nanomed.7, 1805–1818.

    CAS  Google Scholar 

  22. L. Wang, C. C. Liu, Y. Yan Wang, H. Xu, H. Su, and X. Cheng (2016). Curr. Appl. Phys.16, 969–973.

    Article  Google Scholar 

  23. C. Perez, M. Paul, and P. Bazerque (1990). Acta Biol. Med. Exp.15, 113–135.

    Google Scholar 

  24. CLSI, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically (2012) 32, 69.

  25. A. Sankaranarayanan, G. Munivel, G. Karunakaran, S. Kadaikunnan, N. S. Alharbi, N. J. M. Khaled, and D. Kuznetsov (2017). J. Clust. Sci.28, 995–1008.

    Article  CAS  Google Scholar 

  26. B. Malaikozhundan, B. Vaseeharan, S. Vijayakumar, R. Sudhakaran, N. Gobi, and G. Shanthini (2016). Biocatal. Agric. Biotechnol.8, 189–196.

    Article  Google Scholar 

  27. S. Favre-Bonte, T. Kohler, and C. J. Van Delden (2003). Antimicrob. Chemother.52, 598–604.

    Article  CAS  Google Scholar 

  28. D. G. Davies, M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg (1998). Science.280, 295–298.

    Article  CAS  PubMed  Google Scholar 

  29. K. Kalishwaralal, V. Deepak, S. R. Pandian, M. Kottaisamy, S. BarathManiKanth, B. Kartikeyan, and S. Gurunathan (2010). Colloids Surf. B.77, 257–262.

    Article  CAS  Google Scholar 

  30. S. K. Balavandy, K. Shameli, and Z. Z. Abidin (2015). Int. J. Electrochem. Sci.10, 486–497.

    CAS  Google Scholar 

  31. S. K. R. Namasivayam and E. A. Roy (2013). Int. J. Sci. Res. Pub.3, 1–9.

    Google Scholar 

  32. P. Patel, P. Agarwal, S. Kanawaria, S. Kachhwaha, S. Kothari, in Nanotechnology and Plant Sciences (2015), pp. 271–288.

    Google Scholar 

  33. H. V. Tran, L. Dai Tran, C. T. Ba, H. D. Vu, T. N. Guyen, D. G. Pham, and P. X. Nguyen (2010). Colloids Surf. A Physicochem. Eng. Aspects.360, 32–40.

    Article  CAS  Google Scholar 

  34. A. Wei, W. Sun, W. Qian, Y. Ye, and X. Ma (2009). Carbohydr. Res.344, 2375–2382.

    Article  CAS  PubMed  Google Scholar 

  35. S. D. S. Lima, B. Gullon, A. Cardelle-Cobas, L. M. Brito, K. A. F. Rodrigues, P. V. Quelemes, J. Ramos-Jesus, D. D. R. Arcanjo, A. Placido, K. Batziou, P. Quaresma, P. Eaton, C. Delerue-Matos, F. A. A. Carvalho, D. A. Silva, M. Pintado, and J. R. S. A. Leite (2017). J. Bioact. Compat. Polym.32, 397–410.

    Article  CAS  Google Scholar 

  36. P. Kanmani and T. L. Seung (2013). Process Biochem.48, 1099–1106.

    Article  CAS  Google Scholar 

  37. S. Priyadarshini, V. Gopinath, N. Meera Priyadharsshini, D. MubarakAli, and P. Velusamy (2013). Colloid Surf. B.102, 232–237.

    Article  CAS  Google Scholar 

  38. J. S. Kim, J. S. Kuk, K. Yu, J. H. Kim, S. J. Park, H. J. Lee, S. H. Kim, Y. K. Park, Y. H. Park, C. Y. Hwang, Y. K. Kim, Y. S. Lee, D. H. Jeong, and M. H. Cho (2007). Nanomedicine.3, 95–101.

    Article  CAS  PubMed  Google Scholar 

  39. V. Arya, R. Komal, M. Kaur, and A. Goyal (2011). Pharmacologyonline3, 118–124.

    Google Scholar 

  40. E. Borghi, R. Sciota, C. Biassoni, D. Cirasola, L. Cappelletti, L. Vizzini, P. Boracchi, and G. Morace (2011). J. Med. Microbiol.60, 689–690.

    Article  CAS  PubMed  Google Scholar 

  41. S. Manju, B. Malaikozhundan, S. Vijayakumar, S. Shanthi, A. Jaishabanu, P. Ekambaram, and B. Vaseeharan (2016). Microb. Pathog.91, 129–135.

    Article  CAS  PubMed  Google Scholar 

  42. B. Vaseeharan, J. Sivakamavalli, and R. Thaya (2015). J. Compos. Mater.49, 177–184.

    Article  CAS  Google Scholar 

  43. M. M. Kemp, A. Kumar, S. Mousa, T. J. Park, P. Ajayan, N. Kubotera, S. A. Mousa, and R. J. Linhardt (2009). Biomacromolecules.10, 589–595.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. M. Jeyaraj, S. Varadan, K. J. P. Anthony, M. Murugan, A. Raja, and S. Gurunathan (2013). J. Ind. Eng. Chem.19, 1299–1303.

    Article  CAS  Google Scholar 

  45. M. Schäfer and S. Werner (2008). Pharmacol. Res.58, 165–171.

    Article  PubMed  CAS  Google Scholar 

  46. P. Pallavicini, C. R. Arciola, F. Bertoglio, S. Curtosi, G. Dacarro, A. Dagostino, F. Ferrari, D. Merlili, C. Milanese, S. Rossi, A. Taglietti, M. Tenci, and L. Visai (2017). J. Colloid Interface Sci.15, 271–281.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The First author S. Vijayakumar (SRF) thanks the DST, New Delhi, India for financial support under INSPIRE programme (INSPIRE Fellow-IF140145). The authors thank the RUSA phase 2.0 Grant [Ref-24-51-2014-U policy] TN Multi-Gen. Department of Education, Government of India. The authors gratefully acknowledge the Alagappa University, University Scientific Instrumentation Centre (USIC) for providing Confocal laser scanning microscopy, XRD and FTIR instrumental facilities to this research. Mr. S. Vijayakumar acknowledges the help of Mrs. Marry, Nurse, Govt. hospital, Karaikudi, Tamil Nadu, India for blood sample collection.

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  1. Sekar Vijayakumar and Ayyanar Parthasarathy are first author equally contributed.

Authors and Affiliations

  1. Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science Campus 6th Floor, Alagappa University, Karaikudi, Tamil Nadu, 630004, India

    Sekar Vijayakumar, Balasubramanian Malaikozhundan, Ayyanar Parthasarathy & Baskaralingam Vaseeharan

  2. Natural Product Biomedical Technology Laboratory, Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do, 24341, Republic of Korea

    Sekar Vijayakumar, Kandasamy Saravanakumar & Myeong-Hyeon Wang

  3. Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram, Dindigul District, 624 302, Tamil Nadu, India

    Balasubramanian Malaikozhundan

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  1. Sekar Vijayakumar
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Vijayakumar, S., Malaikozhundan, B., Parthasarathy, A. et al. Nano Biomedical Potential of Biopolymer Chitosan-Capped Silver Nanoparticles with Special Reference to Antibacterial, Antibiofilm, Anticoagulant and Wound Dressing Material. J Clust Sci 31, 355–366 (2020). https://doi.org/10.1007/s10876-019-01649-x

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