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The peroxisomal NAD+ carrier of Arabidopsis thaliana transports coenzyme A and its derivatives

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Abstract

The peroxisomal protein PXN encoded by the Arabidopsis gene At2g39970 has very recently been found to transport NAD+, NADH, AMP and ADP. In this work we have reinvestigated the substrate specificity and the transport properties of PXN by using a wide range of potential substrates. Heterologous expression in bacteria followed by purification, reconstitution in liposomes, and uptake and efflux experiments revealed that PNX transports coenzyme A (CoA), dephospho-CoA, acetyl-CoA and adenosine 3′, 5′-phosphate (PAP), besides NAD+, NADH, AMP and ADP. PXN catalyzed fast counter-exchange of substrates and much slower uniport and was strongly inhibited by pyridoxal 5′-phosphate, bathophenanthroline and tannic acid. Transport was saturable with a submillimolar affinity for NAD+, CoA and other substrates. The physiological role of PXN is probably to provide the peroxisomes with the essential coenzymes NAD+ and CoA.

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Authors and Affiliations

  1. Department of Biosciences, Biotechnology and Pharmacological Sciences, University of Bari, Via Orabona 4, 70125, Bari, Italy

    Gennaro Agrimi, Annamaria Russo, Ciro Leonardo Pierri & Ferdinando Palmieri

  2. CNR Institute of Biomembranes and Bioenergetics, Via Orabona 4, 70125, Bari, Italy

    Ferdinando Palmieri

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  1. Gennaro Agrimi
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  2. Annamaria Russo
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  3. Ciro Leonardo Pierri
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  4. Ferdinando Palmieri
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Correspondence to Ferdinando Palmieri.

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Agrimi, G., Russo, A., Pierri, C.L. et al. The peroxisomal NAD+ carrier of Arabidopsis thaliana transports coenzyme A and its derivatives. J Bioenerg Biomembr 44, 333–340 (2012). https://doi.org/10.1007/s10863-012-9445-0

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  • DOI: https://doi.org/10.1007/s10863-012-9445-0

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