Abstract
A profile of free fatty acid (FFA) specificity in Acanthamoeba castellanii mitochondrial uncoupling is described. The FFA uncoupling specificity was observed as different abilities to stimulate resting respiration, to decrease resting membrane potential, and to decrease oxidative phosphorylation efficiency. Tested unsaturated FFA (C18–20) were more effective as uncouplers and protonophores when compared to tested saturated FFA (C8–18), with palmitic acid (C16:0) as the most active. As FFA efficiency in mitochondrial uncoupling is related to physiological changes of fatty acid composition (and thereby FFA availability) during growth of amoeba cells, it could be a way to regulate the activity of an uncoupling protein and thereby the efficiency of oxidative phosphorylation during a cell life of this unicellular organism.
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Abbreviations
- FFA::
-
free fatty acids
- UCP::
-
uncoupling protein
- AcUCP::
-
uncoupling protein of Acanthamoeba castellanii
- Δψ::
-
mitochondrial membrane potential
- ΔμH+::
-
proton electrochemical gradient
- FCCP::
-
p-trifluoromethoxyphenylhydrazone
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Aleksandra Swida and Małgorzata Czarna contributed equally to this work.
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Swida, A., Czarna, M., Woyda-Płoszczyca, A. et al. Fatty acid efficiency profile in uncoupling of Acanthamoeba castellanii mitochondria. J Bioenerg Biomembr 39, 109–115 (2007). https://doi.org/10.1007/s10863-006-9067-5
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DOI: https://doi.org/10.1007/s10863-006-9067-5