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3D construct of hydroxyapatite/zinc oxide/palladium nanocomposite scaffold for bone tissue engineering

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

The purpose of this study was to produce and characterize Hydroxyapatite/Zinc Oxide/Palladium (HA/0.05 wt% ZnO/0.1 wt% Pd) nanocomposite scaffolds and study their mechanical and antibacterial properties, biocompatibility and bioactivity. The initial materials were developed using sol-gel and precipitation methods. Scaffolds were characterized using atomic absorption analysis (AA), scanning electron microcopy (SEM), energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM), atomic force microscopy (AFM) and Brunauer−EmmeS−Teller (BET) method. Furthermore, the bioactivity of scaffolds in simulated body fluid (SBF) and the interaction of dental pulp stem cells (DPSCs) with the nanocomposite scaffolds were assessed. Our results showed that the HA/ZnO/Pd (H1), HA/ZnO/Pd coated by 0.125 g chitosan (H2) and HA/ZnO/Pd coated by 0.25 g chitosan (H3) scaffolds possess higher compressive strength and toughness and lower microhardness and density compared to the pure HA (H0) scaffolds. Immersion of samples in SBF showed the deposition of apatite on the surface of the scaffolds. The biocompatibility assay indicated lower cell proliferation on the H1, H2 and H3 in comparison to the H0. The antibacterial results obtained show a significant impact by loading Pd/ZnO on HA in the deactivation of microorganisms in vitro.

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Acknowledgements

This work was supported by Iran National Science Foundation, INSF, (Grant No. 95838463). The authors would like to thank gratefully Iran National Science Foundation, INSF, for financial support of the research project.

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Authors and Affiliations

  1. Department of Materials Engineering, School of Engineering, Yasouj University, Yasouj, 75918-74934, Iran

    Fatemeh Heidari & Mina Tavangar

  2. Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Fahimeh Sadat Tabatabaei

  3. Marquette University School of Dentistry, Milwaukee, WI, 53233, USA

    Fahimeh Sadat Tabatabaei & Lobat Tayebi

  4. Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA

    Mehdi Razavi

  5. Department of Materials Science and Engineering, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

    Reza Bazargan Lari

  6. Stony Brook University, School of Dental Medicine, Stony Brook, NY, 11794, USA

    Georgios E. Romanos

  7. Department of Electrical and Computer Engineering, NC State University, Raleigh, NC, 27695, USA

    Daryoosh Vashaee

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  1. Fatemeh Heidari
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  2. Fahimeh Sadat Tabatabaei
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Correspondence to Fatemeh Heidari.

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Heidari, F., Tabatabaei, F.S., Razavi, M. et al. 3D construct of hydroxyapatite/zinc oxide/palladium nanocomposite scaffold for bone tissue engineering. J Mater Sci: Mater Med 31, 85 (2020). https://doi.org/10.1007/s10856-020-06409-2

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