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In vivo immunological properties research on mesenchymal stem cells based engineering cartilage by a dialyzer pocket model

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

As the seed cells, the immune properties of the mesenchymal stem cells are important for the tissue engineering restoring effect. But the in vivo research model is lacking. In the study, based on a dialyzer pocket model, changes in immunological properties and the differentiation of seeded mesenchymal stem cells (MSCs) in collagen hydrogel were studied in muscle and articular cavity implantation, respectively. The results showed that collagen hydrogel can induce MSCs to form cartilage tissue, followed by alteration of immunological properties. In muscle implantation, relatively low expression of major histocompatibility complex (MHC) molecules and low level of one-way mixed lymphocyte reactions (MLR) on the seeded MSCs were observed, but only a little cartilage tissue formed. In articular cavity implantation, more cartilage tissue formed, but higher MHC expressions and MLR level were found. Results indicated that the immunomodulation and the cartilage formation of the seeded MSCs will be impacted by the scaffold and the environment of the in vivo implanted site. The dialyzer pocket model can be used for the in vivo research for the MSC-based strategy of the tissue engineering, especially for the optimization of the immunomodulation.

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References

  1. Steinmetz NJ, Aisenbrey EA, Westbrook KK, Qi HJ, Bryant SJ. Mechanical loading regulates human MSC differentiation in a multi-layer hydrogel for osteochondral tissue engineering. Acta Biomater. 2015;21:142–53.

    Article  Google Scholar 

  2. Lavrentieva A, Hatlapatka T, Neumann A, Weyand B, Kasper C. Potential for osteogenic and chondrogenic differentiation of MSC. Adv Biochem Eng Biotechnol. 2012;129:73–88.

    Google Scholar 

  3. Snyder J, Rin Son A, Hamid Q, Wang C, Lui Y, Sun W. Mesenchymal stem cell printing and process regulated cell properties. Biofabrication. 2015;7:044106.

    Article  Google Scholar 

  4. Zhao CY, Zhu XD, Yuan T, Fan HS, Zhang XD. Fabrication of biomimetic apatite coating on porous titanium and their osteointegration in femurs of dogs. Materials Science & Engineering C-Materials for Biological Applications. 2010;30:98–104.

    Article  Google Scholar 

  5. Zheng L, Fan HS, Sun J, Chen XN, Wang G, Zhang L, Fan YJ, Zhang XD. Chondrogenic differentiation of mesenchymal stem cells induced by collagen-based hydrogel: An in vivo study. J Biomed Mater Res A. 2010;93:783–92.

    Google Scholar 

  6. Mamidi MK, Das AK, Zakaria Z, Bhonde R. Mesenchymal stromal cells for cartilage repair in osteoarthritis. Osteoarthritis Cartilage. 2016;24:1307–16.

    Article  Google Scholar 

  7. Saulnier N, Viguier E, Perrier-Groult E, Chenu C, Pillet E, Roger T, Maddens S, Boulocher C. Intra-articular administration of xenogeneic neonatal Mesenchymal Stromal Cells early after meniscal injury down-regulates metalloproteinase gene expression in synovium and prevents cartilage degradation in a rabbit model of osteoarthritis. Osteoarthritis Cartilage. 2015;23:122–33.

    Article  Google Scholar 

  8. Inoue S, Popp FC, Koehl GE, Piso P, Schlitt HJ, Geissler EK, Dahlke MH. Immunomodulatory effects of mesenchymal stem cells in a rat organ transplant model. Transplantation. 2006;81:1589–95.

    Article  Google Scholar 

  9. Liu H, Kemeny DM, Heng BC, Ouyang HW, Melendez AJ, Cao T. The immunogenicity and immunomodulatory function of osteogenic cells differentiated from mesenchymal stem cells. J Immunol. 2006;176:2864–71.

    Article  Google Scholar 

  10. DelaRosa O, Dalemans W, Lombardo E. Mesenchymal stem cells as therapeutic agents of inflammatory and autoimmune diseases. Curr Opin Biotechnol. 2012;23:978–83.

    Article  Google Scholar 

  11. Bassi EJ, de Almeida DC, Moraes-Vieira PM, Camara NO. Exploring the role of soluble factors associated with immune regulatory properties of mesenchymal stem cells. Stem Cell Rev. 2012;8:329–42.

    Article  Google Scholar 

  12. Klinker MW, Wei CH. Mesenchymal stem cells in the treatment of inflammatory and autoimmune diseases in experimental animal models. World J Stem Cells. 2015;7:556–67.

    Article  Google Scholar 

  13. Doorn J, Moll G, Le Blanc K, van Blitterswijk C, de Boer J. Therapeutic applications of mesenchymal stromal cells: paracrine effects and potential improvements. Tissue Eng Part B Rev. 2012;18:101–15.

    Article  Google Scholar 

  14. Cho KS, Kim YW, Kang MJ, Park HY, Hong SL, Roh HJ. Immunomodulatory Effect of Mesenchymal Stem Cells on T Lymphocyte and Cytokine Expression in Nasal Polyps. Otolaryngol Head Neck Surg. 2014;150:1062–70.

    Article  Google Scholar 

  15. Kitazawa Y, Li XK, Xie L, Zhu P, Kimura H, Takahara S. Bone marrow-derived conventional, but not cloned, mesenchymal stem cells suppress lymphocyte proliferation and prevent graft-versus-host disease in rats. Cell Transplant. 2012;21:581–90.

    Article  Google Scholar 

  16. Park MJ, Shin JS, Kim YH, Hong SH, Yang SH, Shin JY, Kim SY, Kim B, Kim JS, Park CG. Murine mesenchymal stem cells suppress T lymphocyte activation through IL-2 receptor alpha (CD25) cleavage by producing matrix metalloproteinases. Stem Cell Rev. 2011;7:381–93.

    Article  Google Scholar 

  17. Yuan T, Li K, Guo L, Fan H, Zhang X. Modulation of immunological properties of allogeneic mesenchymal stem cells by collagen scaffolds in cartilage tissue engineering. J Biomed Mater Res A. 2011;98:332–41.

    Article  Google Scholar 

  18. Bidarra SJ, Barrias CC, Barbosa MA, Soares R, Amedee J, Granja PL. Phenotypic and proliferative modulation of human mesenchymal stem cells via crosstalk with endothelial cells. Stem Cell Res. 2011;7:186–97.

    Article  Google Scholar 

  19. Yuan T, Luo H, Tan J, Fan H, Zhang X. The effect of stress and tissue fluid microenvironment on allogeneic chondrocytes in vivo and the immunological properties of engineered cartilage. Biomaterials. 2011;32:6017–24.

    Article  Google Scholar 

  20. Zheng L, Sun J, Chen X, Wang G, Jiang B, Fan H, Zhang X. In vivo cartilage engineering with collagen hydrogel and allogenous chondrocytes after diffusion chamber implantation in immunocompetent host. Tissue Eng Part A. 2009;15:2145–53.

    Article  Google Scholar 

  21. Cairns DM, Lee PG, Uchimura T, Seufert CR, Kwon H, Zeng L. The role of muscle cells in regulating cartilage matrix production. J Orthop Res. 2010;28:529–36.

    Google Scholar 

  22. Marwood S, Bowtell JL. Effects of glutamine and hyperoxia on pulmonary oxygen uptake and muscle deoxygenation kinetics. Eur J Appl Physiol. 2007;99:149–61.

    Article  Google Scholar 

  23. Lee RB, Wilkins RJ, Razaq S, Urban JP. The effect of mechanical stress on cartilage energy metabolism. Biorheology. 2002;39:133–43.

    Google Scholar 

  24. Fermor B, Christensen SE, Youn I, Cernanec JM, Davies CM, Weinberg JB. Oxygen, nitric oxide and articular cartilage. Eur Cell Mater. 2007;13:56–65. discussion

    Article  Google Scholar 

  25. Gibson JS, Milner PI, White R, Fairfax TP, Wilkins RJ. Oxygen and reactive oxygen species in articular cartilage: modulators of ionic homeostasis. Pflugers Arch. 2008;455:563–73.

    Article  Google Scholar 

  26. Ng KW, DeFrancis JG, Kugler LE, Kelly TA, Ho MM, O’Conor CJ, Ateshian GA, Hung CT. Amino acids supply in culture media is not a limiting factor in the matrix synthesis of engineered cartilage tissue. Amino Acids. 2008;35:433–8.

    Article  Google Scholar 

  27. Hardmeier R, Redl H, Marlovits S. Effects of mechanical loading on collagen propeptides processing in cartilage repair. J Tissue Eng Regen Med. 2009;4:1–11.

    Google Scholar 

  28. Thakkar S, Ghebes CA, Ahmed M, Kelder C, van Blitterswijk CA, Saris D, Fernandes HA, Moroni L. Mesenchymal stromal cell-derived extracellular matrix influences gene expression of chondrocytes. Biofabrication. 2013;5:025003.

    Article  Google Scholar 

  29. Tortorella D, Gewurz B, Schust D, Furman M, Ploegh H. Down-regulation of MHC class I antigen presentation by HCMV; lessons for tumor immunology. Immunol Invest. 2000;29:97–100.

    Article  Google Scholar 

  30. Chamuleau ME, Ossenkoppele GJ, van de Loosdrecht AA. MHC class II molecules in tumour immunology: prognostic marker and target for immune modulation. Immunobiology. 2006;211:619–25.

    Article  Google Scholar 

  31. Triantafillopoulos IK, Papagelopoulos PJ, Politi PK, Nikiforidis PA. Articular changes in experimentally induced patellar trauma. Knee Surg Sports Traumatol Arthrosc. 2002;10:144–53.

    Article  Google Scholar 

  32. Redman SN, Dowthwaite GP, Thomson BM, Archer CW. The cellular responses of articular cartilage to sharp and blunt trauma. Osteoarthritis Cartilage. 2004;12:106–16.

    Article  Google Scholar 

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Acknowledgements

This study is supported by the National Natural Science Foundation of China: 81371685 and 31100692, National key research and development programs of China: 2016YFC1103200.

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Authors and Affiliations

  1. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China

    Tun Yuan, Hongrong Luo, Likun Guo, Hongsong Fan, Jie Liang, Yujiang Fan & Xingdong Zhang

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  1. Tun Yuan
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  2. Hongrong Luo
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  3. Likun Guo
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  4. Hongsong Fan
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  5. Jie Liang
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  6. Yujiang Fan
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Correspondence to Jie Liang or Yujiang Fan.

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Yuan, T., Luo, H., Guo, L. et al. In vivo immunological properties research on mesenchymal stem cells based engineering cartilage by a dialyzer pocket model. J Mater Sci: Mater Med 28, 150 (2017). https://doi.org/10.1007/s10856-017-5955-y

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  • DOI: https://doi.org/10.1007/s10856-017-5955-y

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