Skip to main content

Advertisement

SpringerLink
Log in

Preparation and characterisation of Punica granatum pericarp aqueous extract loaded chitosan-collagen-starch membrane: role in wound healing process

  • Biocompatibility Studies
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Engineered scaffolds made from natural biomaterials are crucial elements in tissue engineering strategies. In this study, biological scaffold like chitosan-collagen-starch membrane (CCSM) loaded with the antibacterial agent, Punica granatum pericarp aqueous extract was explored for enhanced regeneration of epithelial tissue during wound healing. Collagen was extracted from Rachycentron canadum fish skin. Membranous scaffold was prepared by mixing collagen, starch and chitosan in a fixed proportion, loaded with aqueous extract of P. granatum and its anti-pseudomonal activity was studied. Morphological characterization by SEM and mechanical property like tensile strength of the membrane were studied. Excision wound of 2 cm2 size was induced in Guinea pig and the effect of P. granatum extract loaded CCSM in wound healing was studied. The SEM image showed deep pores in the membrane and also possessed good tensile strength. Wound surface area was reduced prominently in the experimental group with P. granatum extract loaded CCSM when compared to the group with unloaded membrane and the one with no membrane. Punica granatum extract loaded CCSM has antipseudomonal property and supported enhanced epithelial cell proliferation without leaving a scar after wound healing. This has significant therapeutic application in membranous scaffold mediated skin repair and regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Caetano GF, Frade MA, Andrade TA, Leite MN, Bueno CZ, Moraes AM, Ribeiro-Paes JT. Chitosan-alginate membranes accelerate wound healing. J Biomed Mater Res B Appl Biomater. 2014;. doi:10.1002/jbm.b.33277.

    Google Scholar 

  2. Guo S, DiPietro LA. Factors affecting wound healing. J Dent Res. 2010;89:219–29.

    Article  Google Scholar 

  3. Phan D, Debeaufort F, Luu D, Voilley A. Moisture barrier, wetting and mechanical properties of shellac agar or shellaccassava starch bilayer bio-membrane for food applications. J Membr Sci. 2008;325:277–83.

    Article  Google Scholar 

  4. Krajewska B, Olech A. Pore structure of gel chitosan membranes. J Mol Catal B. 2000;47:37–47.

    Google Scholar 

  5. Singh P, Benjakul S, Maqsood S, Kishimura H. Isolation and characterization of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). J Food Chem. 2011;124:97–105.

    Article  Google Scholar 

  6. Malafaya PB, Stappers F, Reis RL. Starch-based microspheres produced by emulsion cross linking with a potential media dependent responsive behavior to be used as drug delivery carriers. J Mater Sci. 2006;17:371–7.

    Google Scholar 

  7. Bhowmik D, Duraivel BS, Gopinath H, Kumar BP, Aravind KP, Sampath Kumar KP. Medicinal uses of Punica granatum and its health benefits. J Pharmacogn Phytochem. 2013;1(5):28–35.

    Google Scholar 

  8. Henwood CJ, Livermore DM, James D, Warner M. Antimicrobial susceptibility of Pseudomonas aeruginosa: results of a UK survey and evaluation of the British Society for Antimicrobial Chemotherapy disc susceptibility test. J Antimicrob Chemother. 2001;47:789–99.

    Article  Google Scholar 

  9. Rao YR, Pantulu G, Narayana L. Reduction of fatty acid methyl esters with sodium borohydride-t-butanol-methanol. Eur J Lipid Sci Technol. 2006;89:398–400.

    Google Scholar 

  10. Matsuyama H, Teramoto M, Uesaka T. Membrane formation and structure development by dry-cast process. J Membr Sci. 1997;135:271–88.

    Article  Google Scholar 

  11. Ateyyat MA, Al-Mazra’awi M, Abu-Rjai T, Shatnawi MA. Aqueous extracts of some medicinal plants are as toxic as Imidacloprid to the sweet potato whitefly Bemisiatabaci. J Insect Sci. 2009;9:15.

    Article  Google Scholar 

  12. Zaidan MR, Noor Rain RA, Badrul A, Adlin A, Norazah A, Zakiah I. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. J Trop Med. 2005;22:165–70.

    Google Scholar 

  13. Murthy S, Gautam MK, Goel S, Purohit V, Sharma H, Goel K. Evaluation of in vivo wound healing activity of Bacopamonniera on different wound model in Rats. J Biomed Res Int. 2013;9:9.

    Google Scholar 

  14. Parenteau-Bareil R, Gauvin R, Berthod F. Collagen-based biomaterials for tissue engineering applications. Materials. 2010;3:1863–87.

    Article  Google Scholar 

  15. Attaway H, Gooding CH, Schmidt MG. Comparison of microporous and nonporous membrane bioreactor systems for the treatment of BTEX in vapor streams. J Ind Microbiol Biotechnol. 2002;28:245–51.

    Article  Google Scholar 

  16. Jayakumar R, Menon D, Manzoor K, Nair V, Tamura H. Biomedical applications of chitin and chitosan based nanomaterials. J Carbohydr Polym. 2010;28:142–50.

    Google Scholar 

  17. Bacakova L, Novotna K, Parizek M. Polysaccharides as cell carriers for tissue engineering: the use of cellulose in vascular wall reconstruction. Physiol Res. 2014;63(1):S29–47.

    Google Scholar 

  18. Lin YC, Tan FJ, Marra KG, Jan SS, Liu DC. Synthesis and characterization of collagen/hyaluronan/chitosan composite sponges for potential biomedical applications. Acta Biomater. 2009;5(7):2591–600.

    Article  Google Scholar 

  19. Tuzlakoglu K, Santos MI, Neves N, Reis RL. Design of nano- and microfiber combined scaffolds by electrospinning of collagen onto starch-based fiber meshes: a man-made equivalent of natural extracellular matrix. Tissue Eng Part A. 2011;17:463–73.

    Article  Google Scholar 

  20. Santos MI, Fuchs S, Gomes ME, Unger RE, Reis RL, Kirkpatrick CJ. Response of micro- and macrovascular endothelial cells to starch-based fiber meshes for bone tissue engineering. Biomaterials. 2007;28:240–8.

    Article  Google Scholar 

  21. Santos TC, Morton TJ, Moritz M, Pfeifer S, Reise K, Marques AP, Castro AG, Reis RL, Van Griensven M. Vascular endothelial growth factor and fibroblast growth factor-2 incorporation in starch-based bone tissue-engineered constructs promote the in vivo expression of neovascularization mediators. Tissue Eng Part A. 2013;19:834–48.

    Article  Google Scholar 

  22. Marques AP, Reis RL, Hunt JA. An in vivo study of the host response to starch-based polymers and composites subcutaneously implanted in rats. Macromol Biosci. 2005;5:775–85.

    Article  Google Scholar 

  23. Lin HY, Chen HH, Chang SH, Ni TS. Pectin-chitosan-PVA nanofibrous scaffold made by electrospinning and its potential use as a skin tissue scaffold. J Biomater Sci Polym Ed. 2013;24(4):470–84.

    Article  Google Scholar 

  24. Cahill D, Zhang X, Coronell DG, Mariñas J. Absorption of water in the active layer of reverse osmosis membranes. J Membr Sci. 2009;331:143–51.

    Article  Google Scholar 

  25. Dinu MV, Perju M, Cazacu M, Dragan E. Polyacrylamide-dextran polymeric networks: effect of gel preparation temperature on their morphology and swelling properties. Cell Chem Technol. 2010;45:197–203.

    Google Scholar 

  26. Al Laham S. The anti-bacterial effect of punica granatum extracts against antibiotic resistant pasteurellaha emolytica. J Microbiol. 2013;6:e7750.

    Google Scholar 

  27. Chilukuri DM, Shah JC. Local delivery of vanomycin for the prophylaxis of prosthetic device-related infections. Pharm Res. 2005;22:563–72.

    Article  Google Scholar 

  28. Halim A, Hoo TK, Yusof S. Biologic and synthetic skin substitutes. Ind J Plast Surg. 2010;43:23–8.

    Article  Google Scholar 

  29. Pennington E, Reynolds HY, Carbone P. Pseudomonas pneumonia: a retrospective study of 36 cases. Am J Med. 1973;55:155–60.

    Article  Google Scholar 

  30. Watters C, DeLeon K, Trivedi U, Griswold JA, Lyte M, Hampel KJ, Rumbaugh KP. Pseudomonas aeruginosa biofilms perturb wound resolution and antibiotic tolerance in diabetic mice. Med Microbiol Immunol. 2013;202(2):131–41.

    Article  Google Scholar 

  31. Martin P. Wound healing-aiming for perfect skin regeneration. Science. 1997;276:75–81.

    Article  Google Scholar 

  32. Yan H, Peng KL, Wang QL, Gu ZY, Lu YQ, Zhao J, Xu F, Liu YL, Tang FM, Deng Y, Zhou P. Effect of pomegranate peel polyphenol gel on cutaneous wound healing in alloxan-induced diabetic rats. Chin Med J. 2013;126:1700–6.

    Google Scholar 

  33. Requicha JF, Moura T, Leonor IB, Martins T, Muñoz F, Reis RL, Gomes ME, Viegas CA. Evaluation of a starch-based double layer scaffold for bone regeneration in a rat model. J Orthop Res. 2014;32:904–9.

    Article  Google Scholar 

  34. Choi J-G, Kang O-H, Lee Y-S, Chae H-S, Oh Y-C, Brice O-O, Kim M-S, Sohn D-H, Kim H-S, Park H, Shin D-W, Rho J-R, Kwon D-Y. In vitro and in vivo antibacterial activity of Punica granatum peel ethanol extract against Salmonella. Evid Based Compl Alt. 2011;2011:1–8.

    Google Scholar 

Download references

Acknowledgments

We acknowledge Ms. Anju, Research Scholar, IIUCN, Mahatma Gandhi University, Kerala, India for helping in SEM and tensile strength analyses.

Author information

Authors and Affiliations

  1. Department of Biotechnology, C.M.S College, Kottayam, Kerala, India

    B. Amal

  2. Department of Zoology, S.N College, Cherthala, Kerala, India

    B. Veena

  3. Antibacterials and Microbial Technology Research Lab, Pushpagiri Institute of Medical Science and Research Centre, Thiruvalla, 689101, Kerala, India

    V. P. Jayachandran

  4. Regenerative Medicine and Nanotechnology Lab, Pushpagiri Institute of Medical Sciences and Research Centre, Tiruvalla, 689101, Kerala, India

    Joy Shilpa

Authors
  1. B. Amal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Veena
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. V. P. Jayachandran
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joy Shilpa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joy Shilpa.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Amal, B., Veena, B., Jayachandran, V.P. et al. Preparation and characterisation of Punica granatum pericarp aqueous extract loaded chitosan-collagen-starch membrane: role in wound healing process. J Mater Sci: Mater Med 26, 181 (2015). https://doi.org/10.1007/s10856-015-5515-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10856-015-5515-2

Keywords

Search

Navigation