Skip to main content

Advertisement

SpringerLink
Log in

Metallic ion content and damage to the DNA in oral mucosa cells patients treated dental implants

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

An Erratum to this article was published on 25 May 2014

Abstract

The aim of this study was to assess the potential genotoxicity of dental implants, evaluating biomarkers of DNA damage (micronuclei and/or nuclear buds), cytokinetic defects (binucleated cells) and the presence of trace metals in gingival cells of patients with implants, comparing these with a control group. A total of 60 healthy adults (30 patients with dental implants and 30 control patients without) were included in the study. Medical and dental histories were made for each including life-style factors. Genotoxicity effects were assessed by micronucleus assays in the gingival epithelial cells of each patient; 1,000 epithelial cells were analyzed, evaluating the frequency of micronucleated cells and other nuclear anomalies. The concentration of metals (Al27, Ag107, Co 59, Cr 52, Cu63, Fe56, Sn118, Mn55, Mo92, Ni60, Pb208, Ti47) were assayed by means of coupled plasma-mass spectrophotometry (ICP-MS). The frequency of micronuclei in the patient group with implants was higher than in the control group but without statistically significant differences (P > 0.05). Similar results were found for binucleated cells and nuclear buds (P > 0.05). For metals assayed by ICP-MS, significant differences were found for Ti47 (P ≤ 0.045). Univariate analysis identified a significant association between the presence of micronuclei and age. Dental implants do not induce DNA damage in gingival cells, the slight effects observed cannot be indicated as biologically relevant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Faggion CM Jr, Atieh MA, Park S. Search strategies in systematic reviews in periodontology and implant dentistry. J Clin Periodontol. 2013;40(9):883–8.

    Article  Google Scholar 

  2. Sjöström T. Brydone AS, Meek RM, Dalby MJ, Su B, McNamara LE Titanium nanofeaturing for enhanced bioactivity of implanted orthopedic and dental devices. Nanomedicine (Lond). 2013;8:89–104.

    Article  Google Scholar 

  3. Louropoulou A, Slot DE, Van der Weijden FA. Titanium surface alterations following the use of different mechanical instruments: a systematic review Clin. Oral Impl Res. 2012;23:643–58.

    Article  Google Scholar 

  4. Velasco-Ortega E, Jos A, Cameán AM, Pato-Mourelo J, Segura-Egea JJ. In vitro evaluation of cytotoxicity and genotoxicity of a commercial titanium alloy for dental implantology. Mutation Res. 2010;702:17–23.

    Article  Google Scholar 

  5. Valko M, Morris H, Cronin MT. Metals, toxicity and oxidative stress. Curr Med Chem. 2005;12:1161–208.

    Article  Google Scholar 

  6. Simon M, Lagneau J, Moreno M, Lissac F, Dalard B. Grosgogeat Corrosion resistance and biocompatibility of a new porous surface for titanium implants Eur. J Oral Sci. 2005;113:537–45.

    Article  Google Scholar 

  7. Ribeiro DA, Matsumoto MA, Padovan LE, Marques ME, Salvadori DM. Genotoxicity of corrosion eluates obtained from endosseous implants. Implant Dent. 2007;16:101–9.

    Article  Google Scholar 

  8. Squier CA, Kremer MJ. Biology of oral mucosa and esophagus. J Natl Cancer Inst Monogr. 2001;29:7–15.

    Article  Google Scholar 

  9. Olmedo DG, Paparella ML, Spielberg M, Brandizzi D, Guglielmotti MB, Cabrini RL. Oral mucosa tissue response to titanium cover screws. J Periodontol. 2012;83(8):973–80.

    Article  Google Scholar 

  10. Olmedo DG, Nalli G, Verdú S, Paparella ML, Cabrini RL. Exfoliative cytology and titanium dental implants: a pilot study. J Periodontol. 2013;84:78–83.

    Article  Google Scholar 

  11. Sicilia A, Cuesta S, Coma G, Arregui I, Guisasola C, Ruiz E, Maestro A. Titanium allergy in dental implant patients: a clinical study on 1500 consecutive patients. Clin Oral Impl Res. 2008;19:823–35.

    Article  Google Scholar 

  12. Flatebø RS, Johannessen AC, Grønningsaeter AG, Bøe OE, Gjerdet NR, Grung B, Leknes KN. Host response to titanium dental implant placement evaluated in a human oral model. J Periodontol. 2006;77:1201–10.

    Article  Google Scholar 

  13. Piozzi R, Araki D, Marques LE, Nary H, Akemi M. Genotoxicity and cytotoxicity in multiple organs induced by titanium miniplates in Wistar rats. J Biomed Mater Res. 2008;88:342–7.

    Google Scholar 

  14. Schmalz G, Garhammer P. Biological interactions of dental cast alloys with oral tissues. Dent Mat. 2002;18:396–406.

    Article  Google Scholar 

  15. ADA Council on Scientific Affairs. Titanium applications in dentistry. J Am Dent Assoc. 2003;134:347–9.

    Article  Google Scholar 

  16. Tomakidi P, Koke U, Kern R, Erdinger L, Krüger H, Kohl A, et al. Assessment of acute cyto- and genotoxicity of corrosion eluates obtained from orthodontic materials using monolayer cultures of immortalized human gingival keratinocytes. J Orofac Orthop. 2000;61:2–19.

    Article  Google Scholar 

  17. Ortiz AJ, Fernandez E, Vicente A, Calvo JL, Ortiz C. Metallic ions released from stainless steel, nickel-free, and titanium orthodontic alloys: toxicity and DNA damage. Am J Orthod Dentofac Orthop. 2011;140:e115–22.

    Article  Google Scholar 

  18. Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, Fenech M. Buccal micronucleus cytome assay. Nat Protoc. 2009;4:825–37.

    Article  Google Scholar 

  19. Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E, Knasmueller S, et al. The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN project perspective on current status and knowledge gaps. Mutat Res. 2008;659:93–108.

    Article  Google Scholar 

  20. Bonassi M, Fenech C, Lando YP, Lin M, Ceppi WP, Chang N, et al. Human micronucleus project: international database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes. I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei. Environ Mol Mutagen. 2001;37:31–45.

    Article  Google Scholar 

  21. Sanchez-Siles M, Ros-Llor I, Camacho-Alonso F, Lopez-Jornet P. A novel application of the buccal micronucleus cytome assay in oral lichen planus: a pilot study. Arch Oral Biol. 2011;56:1148–53.

    Article  Google Scholar 

  22. Barnett YA, King CM. An investigation of antioxidants status, DNA repair capacity and mutation as a function of age in humans. Mutat Res. 1995;338:115–28.

    Article  Google Scholar 

  23. Fenech M, Holland N, Zeiger E, Chang WP, Burgaz S, Thomas P, Bolognesi C, Knasmueller S, Kirsch-Volders M, Bonassi S. The HUMN and HUMNxL international collaboration projects on human micronucleus assays in lymphocytes and buccal cells-past, present and future. Mutagenesis. 2011;26:239–45.

    Article  Google Scholar 

  24. Migliore L, Parrini M, Sbrana I, Biagini C, Battaglia A, Loprieno N. Micronucleated lymphocytes in people occupationally exposed to potential environmental contaminants: the age effect. Mutat Res. 1991;256:13–20.

    Article  Google Scholar 

  25. Milosevic-Djordjevic O, Grujicic D, Novakovic T, Arsenijevic S, Marinkovic D. Micronuclei and ageing in a sample of Yugoslavian population. Russ J Genet (Genetika). 2002;37:201–4.

    Article  Google Scholar 

  26. Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E. HUMN project: detailed description of the scoring criteria for the cytokinesis block micronucleus assay using isolated human lymphocyte cultures. Mutat Res. 2003;534:65–75.

    Article  Google Scholar 

  27. Bonassi S, Znaor A, Ceppi M, et al. An increase micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis. 2007;28:625–31.

    Article  Google Scholar 

  28. Sarto F, Finotto S, Giacomelli L, Mazzotti D, Tomanin R, Levis AG. The micronucleus assay in exfoliated cells of the human buccal mucosa. Mutagenesis. 1987;2(1):11–7.

    Article  Google Scholar 

  29. Garhammer P, Schmalz G, Hiller KA, Reitinger T. Metal content of biopsies adjacent to dental cast alloys. Clin Oral Invest. 2003;7:92–7.

    Article  Google Scholar 

  30. Baričević M, Ratkaj I, Mladinić M, Zelježić D, Kraljević SP, Lončar B, Stipetić MM. In vivo assessment of DNA damage induced in oral mucosa cells by fixed and removable metal prosthodontic appliances. Clin Oral Invest. 2012;16:325–31.

    Article  Google Scholar 

  31. Di Pietro A, Visalli G, La Maestra S, Micale R, Baluce B, Matarese G, Cingano L, Scoglio ME. Biomonitoring of DNA damage in peripheral blood lymphocytes of subjects with dental restorative fillings. Mutat Res. 2008;650:115–22.

    Article  Google Scholar 

  32. Piatelli A, Scarano A, Piatelli M, Bertolai R, Panzoni E. Histologic aspects of the bone and soft tissues surrounding three titanium non-submerged plasma-sprayed implants retrieved at autopsy: a case report. J Periodontol. 1997;68:694–700.

    Article  Google Scholar 

  33. de Souza Matos. R, Macedo de Menezes L. Nickel, chromium and iron levels in saliva of patients with simulated fixed orthodontic appliances. Angle Orthod. 2008;78:345–50.

    Article  Google Scholar 

  34. Eliades T, Trapalis C, Eliades G, Katsavarias E. Salivary metal levels of orthodontic patients: a novel methodological and analytical approach. Eur J Orthod. 2003;25:103–6.

    Article  Google Scholar 

  35. Mikulewicz M, Chojnacka K. Trace metal release from orthodontic appliances by in vivo studies: a systematic literature review. Biol Trace Elem Res. 2010;137:127–38.

    Article  Google Scholar 

Download references

Conflict of interest

The authors declare no conflicts of interest

Author information

Authors and Affiliations

  1. Oral Medicine Department, Faculty of Medicine and Dentistry, Ageing Research Institute, University of Murcia, Murcia, Spain

    Pía López-Jornet, Francisco Parra Perrez & Irene LLor-Ros

  2. Department of Implant Dentistry, School of Medicine and Dentistry, University of Murcia, Murcia, Spain

    José Luis Calvo-Guirado & Piedad Ramírez-Fernández

  3. Clínica Odontológica Universitaria, Hospital Morales Meseguer, Adv. Marques de los Velez s/n, 30008, Murcia, Spain

    Pía López-Jornet

Authors
  1. Pía López-Jornet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Francisco Parra Perrez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José Luis Calvo-Guirado
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Irene LLor-Ros
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Piedad Ramírez-Fernández
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pía López-Jornet.

Rights and permissions

Reprints and permissions

About this article

Cite this article

López-Jornet, P., Perrez, F.P., Calvo-Guirado, J.L. et al. Metallic ion content and damage to the DNA in oral mucosa cells patients treated dental implants. J Mater Sci: Mater Med 25, 1819–1824 (2014). https://doi.org/10.1007/s10856-014-5203-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-014-5203-7

Keywords

Search

Navigation